Prof Susan Clark

Lab Head - Epigenetics Research

Senior Principal Research Fellow

Conjoint/Adjunct Role(s)

Conjoint Professor, St Vincent's Clinical School, Faculty of Medicine, UNSW Australia

Susan completed her BSc (Hons1) degree at the Australian National University, ACT, Australia in 1978 and graduated in 1982 with a PhD in Biochemistry, University of Adelaide, South Australia, mapping and sequencing human histone genes, under the supervision of Dr Julian Wells.

Susan spent her postdoctoral years at Biotechnology Australia from 1983-1988 leading studies on recombinant vaccine development and eukaryotic expression of human inhibin, Il-3 and GMCSF. In 1992, she returned to basic research as Group Leader of the Gene Regulation Unit at the Kanematsu Laboratories, Royal Prince Alfred Hospital where she developed and implemented bisulphite sequencing for DNA methylation analysis.  In 2000, she established and headed the Epigenetics Group at the Sydney Cancer Centre, Royal Prince Alfred Hospital and in 2004 moved her group to the Garvan Institute of Medical Research and initiated and led the growth of the Epigenetics Research Program in the Cancer Research Division.  

Her DNA methylation studies over the last twenty years have initiated profound questions about the importance of epigenetics in early development and in disease, especially in cancer.  Susan has made extensive ground-breaking discoveries relating to DNA methylation patterns in normal and cancer genomes, that have led to the commercialization of new methylation-based tests for early cancer detection.  The techniques she pioneered in the early 1990s, including bisulphite sequencing, have revolutionised and now underpin the new era in epigen"omic" research. She was founding member of IHEC (International Human Epigenome Consortium) and led the formation of the AEpiA (Australian Epigenetics Alliance). 

She has a number of awards including the RPAH Research Medal in 2002, Julian Wells Medal in 2003, “Biochemisch Analytik Preis” for outstanding contribution for Methylation analysis in 2004.  In 2006 was elected a Fellow of the World Technology Network for Biotechnology, in 2009 was awarded one of Australia’s "Top Ten" National Health and Medical Research (NHMRC) Project Scientists and the Rotary Award for Vocational Excellence in 2012. 

Research Interests

Bioinformatics
Cancer Research
Chromatin
Disease Mechanisms
DNA methylation
Epigenetics
Gene Expression
Molecular Genetics
Next Generation Sequencing

Awards and Honours

2013 - Senior Principal Research Fellowship (NHMRC)
2012 - Rotary Award for Vocational Excellence
2011 - Victor Chang Medal (Barbara Ell Seminar Series Lecturer)
2010 - Service to Science and Industry, North Shore Times, 50 year medal
2009 - Australia’s “Top Ten” (NHMRC) Scientist Award for 2009
2009 - Promoted Senior Principal Research Fellow (Garvan Institute)
2008 - Principal Research Fellowship (NHMRC)
2008 - Adjunct Professor, Faculty Medicine, University of NSW
2008 - Director’s Inaugural Women in Science Lecture Award (WEHI)
2006 - World Technology Award, finalist for 2006 for Biotechnology: the most innovative people in the science and technology world in 2006
2006 - Elected a Fellow of the World Technology Network for Biotechnology for contribution to Epigenetics technology
2004 - Principal Research Fellowship (NHMRC)
2004 - The Ruby Payne-Scott Award for women in Australian science
2004 - German Science Prize “Biochemisch Analytik Preis” (50,000 Euros) for outstanding contribution for Methylation analysis. Since 1970, this award has recognized the work of 27 world-renowned scientists, four of whom have subsequently gone on to win a Nobel Prize
2003 - Julian Wells Medal for “outstanding contribution to gene action and genome structure”, Lorne Genome conference
2002 - RPAH Research Medal for $50,000 for major contribution to research
1998 - Cancer Council NSW Travelling Award
1997 - DNA Methylation Imprinting and the Epigenetics of Cancer, AACR, Research Presentation Award, Puerto Rico
1978 - Commonwealth Post Graduate Research Award
1976 - CRAC Undergraduate Exchange Travelling Award to study at Cambridge

Education

1982 - PhD, University of Adelaide, South Australia - Australia
1978 - BSc (Hons1) - Australian National University, ACT - Australia
1977 - BSc, Australian National University, ACT - Australia

Selected Publications

Bert SA, Robinson MD, Strbenac D, Statham AL, Song JZ, Hulf T, Sutherland RL, Coolen MW, Stirzaker C and Clark SJ. Regional activation of the cancer genome by long range epigenetic remodelling. Cancer Cell 2013; 23:9-22.

Valdes-Mora F, Song JZ, Statham AL, Strbenac D, Robinson MD, Nair SS, Patterson KI, Tremethick DJ, Stirzaker C and Clark SJ (2012). Acetylation of H2A.Z is a key epigenetic modification associated with gene deregulation and epigenetic remodeling in cancer. Genome Res 2012;22: 307-321.

Statham AL, Robinson MD, Song JZ, Coolen MW, Stirzaker C and Clark SJ. Bisulfite sequencing of chromatin immunoprecipitated DNA (BisChIP-seq) directly informs methylation status of histone-modified DNA. Genome Res 2012;22: 1120-1127.

Coolen MW, Stirzaker C, Song JZ, Statham AL, Kassir Z, Moreno CS, Young AN, Varma V, Speed TP, Cowley M, Lacaze P, Kaplan W, Robinson MD and Clark SJ. Consolidation of the cancer genome into domains of repressive chromatin by long-range epigenetic silencing (LRES) reduces transcriptional plasticity. Nat Cell Biol 2010; 12:235-246.

Hinshelwood RA, Melki JR, Huschtscha LI, Paul C, Song JZ, Stirzaker C, Reddel RR and Clark SJ. Aberrant de novo methylation of the p16(INK4A) CpG island is initiated post gene silencing in association with chromatin remodelling and mimics nucleosome positioning. Hum Mol Genet 2009; 18:3098-3109.

Hinshelwood RA, Huschtscha LI, Melki J, Stirzaker C, Abdipranoto A, Vissel B, Ravasi T, Wells CA, Hume DA, Reddel RR and Clark SJ. Concordant epigenetic silencing of transforming growth factor-beta signaling pathway genes occurs early in breast carcinogenesis. Cancer Res 2007; 67:11517-11527.

Frigola J, Song J, Stirzaker C, Hinshelwood RA, Peinado MA and Clark SJ (2006). Epigenetic remodeling in colorectal cancer results in coordinate gene suppression across an entire chromosome band. Nat Genet 38: 540-549.

Stirzaker C, Song JZ, Davidson B and Clark SJ. Transcriptional gene silencing promotes DNA hypermethylation through a sequential change in chromatin modifications in cancer cells. Cancer Res 2004; 64:3871-3877.

Song JZ, Stirzaker C, Harrison J, Melki JR and Clark SJ. Hypermethylation trigger of the glutathione-S-transferase gene (GSTP1) in prostate cancer cells. Oncogene 2002; 21:1048-1061.

Clark SJ and Melki J. DNA methylation and gene silencing in cancer: which is the guilty party? Oncogene 2002; 21:5380-5387.

Millar DS, Ow KK, Paul CL, Russell PJ, Molloy PL and Clark SJ. Detailed methylation analysis of the glutathione S-transferase pi (GSTP1) gene in prostate cancer. Oncogene 1999; 18:1313-1324.

Melki JR, Vincent PC and Clark SJ. Concurrent DNA hypermethylation of multiple genes in acute myeloid leukemia. Cancer Res 1999; 59:3730-3740.

Stirzaker C, Millar DS, Paul CL, Warnecke PM, Harrison J, Vincent PC, Frommer M and Clark SJ. Extensive DNA methylation spanning the Rb promoter in retinoblastoma tumors. Cancer Res 1997; 57:2229-2237.

Clark SJ, Harrison J and Frommer M. CpNpG methylation in mammalian-cells. Nat Genet 1995; 10:20-27.

Clark SJ, Harrison J, Paul CL and Frommer M. High-sensitivity mapping of methylated cytosines. Nucleic Acids Res 1994; 22:2990-2997.

Prof Susan Clark