Dr Daniel Christ
Senior Research Fellow; Group Leader Immunology Program and Director Antibody Development, Garvan Institute of Medical Research; Conjoint Senior Lecturer Faculty of Medicine, The University of New South Wales
Email: d.christ "at"garvan.org.au
Research Group: Antibody Engineering
After undergraduate studies in Switzerland, Daniel joined Sir Gregory Winter's laboratory at Cambridge University for his PhD. Winter had shown that in-vitro evolution technology (such as phage display) can be used for the generation of therapeutic antibodies, initiating a multi-billion dollar biotechnology industry. At Cambridge, Christ and Winter further developed monoclonal antibody technology, particularly in the field of domain antibodies. He was elected a Fellow of Trinity College (Cambridge University) at age 29. He maintains close links with industry and was involved the foundation of Domantis Ltd, for which he worked as a consultant until its sale to GSK in 2006. Daniel joined Garvan in 2007 to head the newly established antibody engineering lab.
2003 PhD, Cambridge University
1999 Dipl. Natw. (M.Sc.), Swiss Federal Institute of Technology (ETH)
Awards and Honours
2009 NHMRC CDA Fellow
2002 Fellow, Trinity College, Cambridge University
1999 ERS PhD Scholarship, Trinity College, Cambridge University
1999 Studer Prize (best graduate), Swiss Federal Institute of Technology (ETH)
Dudgeon K, Rouet R, Kokmeijer I, Schofield P, Stolp J, Langley D, Stock D and Christ D. (2012) General strategy for the generation of human antibody variable domains with increased aggregation resistance. Proc Natl Acad Sci U S A. 109: 10879-10884. [ Editorial: http://www.pnas.org/content/109/27/10741.full.pdf]
Rouet R, Dudgeon K, Schofield P, Lowe D, Jermutus L and Christ D. (2012) Expression of high affinity antibody fragments in bacteria. Nature Protoc. 7: 364-373.
Lowe D, Dudgeon K, Rouet R, Schofield P, Jermutus L and Christ D. Aggregation, stability, and formulation of human antibody therapeutics. Adv Protein Chem Struct Biol 2011;84:1-206
O'Toole SA, Machalek DA, Shearer RF, Millar EK, Nair R, Schofield P, McLeod D, Cooper CL, McNeil CM, McFarland A, Nguyen A, Ormandy CJ, Qiu MR, Rabinovich B, Martelotto LG, Vu D, Hannigan GE, Musgrove EA, Christ D, Sutherland RL, Watkins DN, Swarbrick A. Hedgehog overexpression is associated with stromal interactions and predicts for poor outcome in breast cancer. Cancer Res 2011 71(11):4002-4014
Lee CM, McGuire H, King C & Christ D. Expression, purification and characterization of recombinant interleukin-21 J. Immunol. Methods 2010; 362 (1-2):185-189.
Famm K, Hansen L, Christ D & Winter G. Thermodynamically stable aggregation-resistant antibody domains through directed evolution. J Mol Biol 2008; 376:926-931.
Lee CM, Iorno N, Sierro F & Christ D. Selection of human antibody fragments by phage display. Nat Protoc 2007; 2(11):3001-3008
Christ D, Famm K, Winter G. Repertoires of aggregation-resistant human antibody domains. Protein Eng Des Sel 2007; 20(8):413-416.
Christ D, Famm K, Winter G. Tapping diversity lost in transformation - in vitro amplification of ligation reactions. Nucl Acids Res 2007; 34(16):e108.
Christ D, Winter G. Identification of protein domains by shotgun proteolysis. J Mol Biol 2006; 358(2):364-371.
Christ D, Winter G. Identification of functional similarities between proteins by directed evolution. Proc Natl Acad Sci U S A 2003; 100(23):13202-13206.
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