Molecular Genetics Prices

Reagent Ordering Form

   

We buy in bulk and get special prices for you.

When we provide you with reagents you profit from bulk pricing and you do not have to pay for delivery costs.

Our reagents are available 24/7 as you can send an email to and let us know what you have used when you work on weekends or after hours. Please do remember to send us this email as this system is based on trust. Alternatively leave a short message on the desk of the Sequencing Service Coordinator with your full name and the reagent or consumable you have taken.

 

Mouse Genotyping Service Prices (External/Internal)

All PCRs are run with internal control housekeeping PCR and a WT control DNA additional to gene specific controls (if provided), these controls are included in the price listed:

external/internal
Mouse Genotyping 1 PCR $4.10 / 3.35 Shop Meltcurve
Mouse Genotyping 2 PCRs $7.20 / 5.20
HRM SNP detection (any SNP also human) $6.00 / 5.00
Copy Number Het/Hom Differentiation. This assay has a risk of false results, this risk can be minimized by submitting a het and a hom control with the samples, (control DNA prepped with the samples). The DNA quality is crucial for this test, we require 50ul of DNA at 50ng/ul (all DNAs diluted to this same concentration). The results of this assay cannot be translated directly in a breeding scheme but must be verified by cross-testing breeding strategy.  $60 / 40
Project establishment for a new PCR (primer design, ordering of primers, testing of PCR with known controls or DNA of animal tissue with known genotypes) $120

 

Cell line Identification Service Prices (External/Internal)

For DNA samples

The Cell line authentication is based on the Powerplex18D PCR. DNA samples are analysed, interpreted and authenticated via our own database. The authentication report is sent via email as a pdf.

$139  Shop Cell Line

For Cell samples 

$149.50

We perform a Mycoplasma test via PCR. We amplify in regions of the 23s and the 16s rRNA and find the follwing species:

Mycoplasma hyorhinis, orale, hominis,  argininis, salivarium,  pirum, fermentans,   and Acholeplasma laidlawii, account for over 95% of isolates from cultured  cells. In addition we are able to detect Mycoplasma pneumoniae, gallisepticum, genitalium,   penetrans, synoviae, bovis, hyopneumoniae,Ureaplasma urealyticum, Spiroplasma citri.

$45.60 Mycoplasma picture

 

 Sequencing & Fragment Separation Service 

Service Sample Price Shop Sequencing
Premix primer and PCR or Plasmid (NATA certified) $5.00

Fragment Separation without size standard

$4.00
Fragment Separation with size standard $5.50

Plasmid Mix & SEQ

(you provide the plasmid DNA, we measure the DNA concentration, dilute to optimal conc. and mix with primer and run Sanger sequencing)

$ 11.50

PCR Mix & SEQ (bidirectional)

(you provide the PCR product, (we cleanup the PCR product, we measure the DNA concentration, dilute to optimal conc. and mix with primer and run Sanger sequencing)

$ 18.50

PCR Setup & SEQ (bidirectional)

(you provide the DNA, we setup & cleanup the PCR product, we measure the DNA concentration, dilute to optimal conc. and mix with primer and run Sanger sequencing)

$ 27.50

PCR Design & SEQ (bidirectional)

(includes primer design, primer order, PCR setup, PCR cleanup, dilution of PCR product, SEQ setup and trace QC )

$ 76.20
Analysis of PCR project per sample (bidirectional) and report (ISO 17025) $ 53.80
Analysis of PCR project per sample (bidirectional) and clinical report (ISO 15189) signed by genetic pathologist upon request
Large PCR projects upon request
Large Clinical Diagnostic Sequencing Projects  upon request

 

Decontamination

DNA away (1x bottle) $45.00
RNA away (1x bottle) $87.00
Nuclease free water from Qiagen (1000ml) $90.00
Please use RNA and DNA away when bringing racks or pipettes in the prePCR room

 

Bioanalyzer 2100 Nano Chips

RNA Nano Chip (incl. reagent)

12 Samples can be run on one chip

$95.00 Bioanalyzer 2100 nano chips

RNA Pico Chip (incl. reagent )

11 Samples can be run on one chip

$120.00

DNA High Sensitivity Chip (incl. reagent )

11 Samples can be run on one chip

$120.00

DNA 1000 Chip (incl. reagent )

12 Samples can be run on one chip

$110.00

 Please see our Document and Links webpage > the Nano, Pico, HS and 1000 operation manuals


Microtiter Plates

ABI plates 10X $115.00 Microtiter Plates
BioRad 96 well plates 5X $30.00
Roche LC480 plates 10X $115.00
Optical seals 10X $35.00


Master Mixes

ABI MM Universal (old) 5ml $400.00 Master Mixes
ABI MM Gene Expression 5ml  $350.00
ABI MM Power SYBR green 5ml $350.00
Sensi dt Bioline MM 1ml $90.00
Sensi dt Bioline MM 5ml $350.00
Roche Probemaster MM 5ml $450.00
MyFi DNA Polymerase Bioline  250units $130.00
MyTaq HS DNA Polymerase Bioline  500units $320.00
SensiFAST Probe No-ROX kit Bioline 1ml $70.00
SensiFAST SYBR No-ROX kit Bioline 1ml $70.00

 

cDNA Synthesis

Tetro cDNA Synthesis kit 100x  $310.00 cDNA Synthesis
MEGAshortscript™ T7 Transcription Kit $736.00
ABI multiscribe reverse transcriptase $185.00

 

Intercalating Dyes

Sito9 vial (500ul) $310.00 Intercalating Dyes
Evagreen (1000ul) $80.00
Resolight from Roche (500ul) $210.00
Gelred (500ul) $145.00
Diamond Nucleic Acid Dye (500ul) $170.00

 Please click on the dye above or see our Document and Links webpage > Geldoc for instructions on how to use Intercalating Dyes

 

Qubit Quantification

RNA HighSensitivity (1 Sample) $1.40 Qubit
DNA HighSensitivity (1 Sample) $1.40
DNA BroadRange (1 Sample) $1.40

 Please see our Document and Links webpage > Qubit for the Qubit Operational Manual

 

3D digital PCR

3D chips (= 1 sample) $15.00

Quant 3 D Mastermix (5ml)

Quant 3 D Mastermix (1.5ml)

$480.00

$180.00

Instrument usage / chip $5.00

 Please see our Document and Links webpage 3D digital PCR for the Operational Manual

 

Lifetechnology (formerly ABI) Assays

ABI Taqman Assay (inventoried) XS

100x 10ul reactions (50ul, use 0.5ul)

$130.00 Lifetechnology (formerly ABI) Assays

ABI Taqman Assay (inventoried) S

500x 10ul reactions (250ul, use 0.5ul)

$255.00

ABI Taqman Assay (inventoried) M

750x 10ul reactions (375ul, use 0.5ul)

$950.00

ABI Custom Made (non-inventoried)  MED

720x 10ul reactions (375ul, use 0.5ul)

$1040.00

ABI Custom Made (non-inventoried)  SMALL

500 x 10ul reactions (250ul, use 0.5ul)

$546.00
TaqMan MGB probes (6,000 pmoles) $650.00
TaqMan® SNP Genotyping Assay, Human SMALL $500.00
TaqMan® SNP Genotyping Assay, Human MED $1000.00
TaqMan® miRNA Assays (inventoried) $294.00
TaqMan® miRNA Assays (non-inventoried) $369.00
ABI Human GAPD (GAPDH) Endogenous Control (FAM™ ⁄ MGB Probe, Non-Primer Limited) $983.00

 

EPmotion Tips

EpMotion tips / card (15 x 96 tips) $280.00 EPmotion Tips

 

Universal Probe Library (UPL)

UPL probe (1 to 165) per reaction (0.1ul probe/10ul mastermix)

$0.45 Universal Probe Library (UPL)
VIC labelled house keeper (0.1ul) $1.20

 

DNA Extraction kits 

DNA extraction 1x column (Midi kit)

$17.25 DNA Prep costs

DNAeasy extraction kit 1x column (Mini kit)

$7.00

DNAinvestigator kit 1x column (Mini kit)

$14.00
Bioline Isolate II Genomic DNA kit (50preps) $12.00

 

DNA/RNA Extraction Service (on QiaSymphony with magnetic beads)

Sample TypeMaterialPrice
DNA Whole Blood (200 ul)                                                          $8.50
DNA Cells $8.50
DNA Fresh Frozen Tissue (excl.homogenization) $8.50
DNA Tissue (incl. homogenization) $28.80
DNA Buffy Coat (200 ul)   $8.50
DNA Buccal Swab (OraCollect) $33.80
RNA (DNAse treated) Whole Blood  $11.67 
RNA (DNAse treated) PAXgene tube (tube provided by client) $23.67
RNA (DNAse treated) FFPE $29.76 
RNA (DNAse treated) Tissue (incl. homogenization) $26.40 
RNA (DNAse treated) Buffy Coat    $11.84    
RNA (DNAse treated) Cells $11.84
DNA/RNA Quality Check (QC) Agilent chip $150/12
DNA/RNA Quality Check (QC) Nanodrop $1.80
DNA/RNA Quality Check (QC) QBit $2.40

DNA/RNA Extraction Service (on QiaCube with spin columns)

Sample TypeMaterialPriceQiagen QiaCube DNA/RNA Extraction
DNA Whole Blood (200 ul)                                                             $14.00
DNA Cells $14.00
DNA Buffy Coat (200 ul)   $14.00
RNA Whole Blood (PAXgene 1 mL) $22.00 
RNA Buffy Coat    $22.00    
RNA Cells $22.00

 

RNA Prep Kit

Bioline Isolate II RNA Mini Kit (50 Preps) $220.00 RNA Prep Kit

 

Primer Ordering Service

Sigma-Aldrich Oligos

Primer Ordering Service
Standard desalted Oligo (25 nmol scale) length 15- 60 nucleotides (delivery 4-5 working days)

$7.00 

no express service avilable anymore
HPLC, PAGE purification (minimum scale is 25nmol) $35.00
Ultramere length 60- 120 nucleotides (4nmol) $1.50/nt
Modified Oligos, 5' or 3' Phos, Biotin, FAM, VIC, etc (100 nmol scale minimum) price on request

IDT

Standard desalted Oligo (25 nmol scale) length 15-34 nucleotides (delivery ~3 working days) $7.00 
Standard desalted Oligo (25 nmol scale) length 10-59 nucleotides $10.00 
Standard desalted Oligo (100 nmol scale) length 10-90 nucleotides $26.00
HPLC, PAGE purification (minimum scale is 100nmol) $45.00
Modified Oligos, 5' or 3' Phos, Biotin, FAM, VIC, etc (100 nmol scale minimum) price on request

Geneworks

Standard desalted Oligo (40 nmol scale) up to 25mers (delivery 4 working days)    $20.00
Express Service (40 nmol scale) up to 25mers (delivery 1 to 2 working days)       $27.00
Modified Oligos, Oligos longer than 25mers or scales higher than 25 nmol price on request

Ultramers from IDT

Ultramer 4nmole (65 - 200 Bp) price per Base $ 1.10
Ultramer 20 nmole (65 - 200 Bp) price per Base $ 2.15
PAGE Ultramer (45 -200Bp) price per Base $ 1.10

gBlocks from IDT

gBlocks® Gene Fragments, 125-500 bp $ 105.00
gBlocks® Gene Fragments, 501-750 bp $ 145.00
gBlocks® Gene Fragments, 751-1000 bp $ 165.00
gBlocks® Gene Fragments, 1001-1250 bp $ 225.00
gBlocks® Gene Fragments, 1251-1500 bp $ 265.00
gBlocks® Gene Fragments, 1501-1750 bp $ 305.00
gBlocks® Gene Fragments, 1751-2000 bp $ 345.00

 

Fluorescent Antibodies for Western Blots and Blocking Buffer (Licor Odyssey)

Aliquots of antibodies contain 5ul (enough for one blot) 

Fluorescing Secondary Antibody Aliquot IRDye 680LT Donkey anti-Mouse  $5.00 Antiboies
Fluorescing Secondary Antibody Aliquot IRDye 800CW Donkey anti-Mouse $5.00
Fluorescing Secondary Antibody Aliquot IRDye 800CW Donkey anti-Rabbit $5.00
Fluorescing Secondary Antibody Aliquot IRDye 680LT Donkey anti-Rabbit $5.00
Fluorescing Secondary Antibody Aliquot IRDye 800CW Donkey anti-Goat  $5.00
Fluorescing Secondary Antibody Aliquot IRDye 680LT Donkey anti-Goat $5.00
Blocking Buffer Licor 1x bottle (500ml) $150.00
Blocking Buffer Licor 50ml Aliquot $17.00

 

High Throuput SNP Detection on Sequenom

Depending on the number of samples and SNPs the price per SNP will vary. But in general we charge a full 384well plate, either filled with 384 different samples and each well can have ~30 different primers (total of 384x30=11520 SNPs) or less samples and more SNPs.

Cost for a run (384 plate minimum)

$1600.00 High throuput SNP detection on Sequenom
+ costs for primers 

depending
on number
of SNPs