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Measuring phospholipase D activity in insulin-secreting pancreatic beta-cells and insulin-responsive muscle cells and adipocytes

Abstract

Phospholipase D (PLD) is an enzyme producing phosphatidic acid and choline through hydrolysis of phosphatidylcholine. The enzyme has been identified as a member of a variety of signal transduction cascades and as a key regulator of numerous intracellular vesicle trafficking processes. A role for PLD in regulating glucose homeostasis is emerging as the enzyme has recently been identified in events regulating exocytosis of insulin from pancreatic beta-cells and also in insulin-stimulated glucose uptake through controlling GLUT4 vesicle exocytosis in muscle and adipose tissue. We present methodologies for assessing cellular PLD activity in secretagogue-stimulated insulin-secreting pancreatic beta-cells and also insulin-stimulated adipocyte and muscle cells, two of the principal insulin-responsive cell types controlling blood glucose levels.

Type Journal
ISBN 1064-3745 (Print)
Authors Cazzolli, R.; Huang, P.; Teng, S.; Hughes, W. E.;
Garvan Authors Dr William Hughes
Publisher Name Methods Mol Biol
Published Date 2009-01-01 00:00:00
Published Volume 462
Published Pages 241-51
URL http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19160674
Status Published In-print
OpenAccess Link https://publications.gimr.garvan.org.au/download.php?10204_10352/09 Cazzoli MMB.pdf