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Selection of human VH single domains with improved biophysical properties by phage display

Abstract

Human antibody variable heavy (VH) domains tend to display poor biophysical properties when expressed in isolation. Consequently, the domains are often characterized by low expression levels, high levels of aggregation, and increased "stickiness." Here, we describe methods that allow the engineering of human VH domains with improved biophysical properties by phage display. The engineered domains withstand challenging conditions, such as high temperature and acidic pH. Engineered human single domains are a promising new class of antibody fragments and represent robust research tools and building blocks for the generation of antibody therapeutics.

Type Journal
ISBN 1940-6029 (Electronic) 1064-3745 (Linking)
Authors Dudgeon, K.; Rouet, R.; Famm, K.; Christ, D.;
Garvan Authors A/Prof Daniel Christ
Publisher Name Methods Mol Biol
Published Date 2012-01-01 00:00:00
Published Volume 911
Published Pages 383-97
URL http://www.ncbi.nlm.nih.gov/pubmed/22886264
Status Published In-print
OpenAccess Link https://publications.gimr.garvan.org.au/download.php?11551_12025/12 Dudgeon Meth Mol Biol_.pdf