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Human C5aR knock-in mice facilitate the production and assessment of anti-inflammatory monoclonal antibodies

Abstract

Complement component C5a binds C5a receptor (C5aR) and facilitates leukocyte chemotaxis and release of inflammatory mediators. We used neutrophils from human C5aR knock-in mice, in which the mouse C5aR coding region was replaced with that of human C5aR, to immunize wild-type mice and to generate high-affinity antagonist monoclonal antibodies (mAbs) to human C5aR. These mAbs blocked neutrophil migration to C5a in vitro and, at low doses, both prevented and reversed inflammatory arthritis in the murine K/BxN model. Of approximately 40 mAbs generated to C5aR, all potent inhibitors recognized a small region of the second extracellular loop that seems to be critical for regulation of receptor activity. Human C5aR knock-in mice not only facilitated production of high-affinity mAbs against an important human therapeutic target but were also useful in preclinical validation of the potency of these antagonists. This strategy should be applicable to other important mAb therapeutics.

Type Journal
ISBN 1087-0156 (Print)
Authors Lee, H.;Zahra, D.;Vogelzang, A.;Newton, R.;Thatcher, J.;Quan, A.;So, T.;Zwirner, J.;Koentgen, F.;Padkjaer, S. B.;Mackay, F.;Whitfeld, P. L.;Mackay, C. R. :
Publisher Name NAT BIOTECHNOL
Published Date 2006-01-01 00:00:00
Published Volume 24
Published Issue 10
Published Pages 1279-84
URL http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16980974
Status Published In-print
OpenAccess Link https://publications.gimr.garvan.org.au/download.php?2075_10579/06 Lee Nature BT.pdf