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Testosterone radioimmunoassay: rapid extraction technique for steroids based on kieselguhr mini-columns

Abstract

A technique for the rapid extraction of steroids from serum prior to their radioimmunoassay is described. The technique is based on the use of mini-columns filled with kieselguhr (Extrelut; Merck). The columns provide a means of achieving a rapid and precise extraction without the problem of emulsion formation and without the necessity to accurately measure organic solvent volumes. The technique is inexpensive, time saving and at least as precise as most conventionally extracted steroid assays. The results achieved correlate well with those of a conventionally extracted assay.

Type Journal
ISBN 0039-128X (Print)
Authors Vining, R. F. :
Publisher Name STEROIDS
Published Date 1980-01-01 00:00:00
Published Volume 36
Published Issue 2
Published Pages 149-59
URL http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=6255632
Status Published In-print