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Selection of human antibody fragments by phage display

Abstract

Here, we describe a protocol for the selection of human antibody fragments using repertoires displayed on filamentous bacteriophage. Antigen-specific clones are enriched by binding to immobilized antigen, followed by elution and repropagation of phage. After multiple rounds of binding selection, specific clones are identified by ELISA. This article provides an overview of phage display and antibody technology, as well as detailed protocols for the immobilization of antigen, the selection of repertoires on purified or complex antigens and the identification of binders.

Type Journal
ISBN 1750-2799 (Electronic)
Authors Lee, C. M.;Iorno, N.;Sierro, F.;Christ, D. :
Garvan Authors A/Prof Daniel Christ
Publisher Name NAT PROTOC
Published Date 2007-01-01 00:00:00
Published Volume 2
Published Issue 11
Published Pages 3001-8
URL http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18007636
Status Published In-print
OpenAccess Link https://publications.gimr.garvan.org.au/download.php?2235_11094/07 Lee nprot.pdf