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Engineering Escherichia coli heat-resistance by synthetic gene amplification

Abstract

Organisms have evolved to exploit new environments by processes that involve both mutations and gene amplifications. Though in some cases amplified genes mutate to perform a different molecular function, in other cases altering gene copy number alone is sufficient to change organism function. Here we selected a library of genes, provided at high copy number, for their ability to confer survival on Escherichia coli cells at un-physiologically high temperatures. We find that a single gene (evgA), encoding a master transcriptional regulator, is overwhelmingly selected and allows survival upon heating to temperatures in excess of 50 degrees C. While the detailed mechanisms of this resistance remained unclear, our results demonstrate the potential of copy number manipulation for the engineering of organisms.

Type Journal
ISBN 1741-0126 (Print)
Authors Christ, D.;Chin, J. W. :
Publisher Name PROTEIN ENG DES SEL
Published Date 2008-01-01 00:00:00
Published Volume 21
Published Issue 2
Published Pages 121-5
URL http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18175777
Status Published In-print
OpenAccess Link https://publications.gimr.garvan.org.au/download.php?2328_10142/08 Christ PEDS.pdf