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Immunochemical detection and characterisation of osteocalcin from moa bone

Abstract

Osteocalcin (the 6,000 dalton Mr gamma-carboxyglutamate-containing protein of bone) has been detected in acid extracts of bones of the extinct class of New Zealand ratite birds, the moas, using a radioimmunoassay for sheep osteocalcin. The immunoreactive osteocalcin of the extracts of two of these bones (the fibulae from two specimens of Pachyornis elephantopus found in South Island swamps) has been fractionated by gel filtration chromatography and reversed-phase high performance liquid chromatography, and behaves in a manner characteristic of osteocalcin from modern bones. Carbon-14 dating of bones and gizzard contents found in association with these specimens indicates approximate ages of 3,600 and 7,400 years respectively.

Type Journal
ISBN 0006-291X (Print)
Authors Huq, N. L.;Rambaud, S. M.;Teh, L. C.;Davies, A. D.;McCulloch, B.;Trotter, M. M.;Chapman, G. E. :
Publisher Name BIOCHEM BIOPH RES CO
Published Date 1985-01-01 00:00:00
Published Volume 129
Published Issue 3
Published Pages 714-20
URL http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=3874623
Status Published In-print