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Localization of human pituitary hormones by multiple immunoenzyme staining procedures using monoclonal and polyclonal antibodies

Abstract

Mouse monoclonal and rabbit polyclonal antibodies to human pituitary hormones were applied together to sections of normal and neoplastic human pituitary tissue. Binding sites were revealed with species-specific immune reagents combined with various enzymes (peroxidase, alkaline phosphatase, and beta-D-galactosidase). The enzymes were developed separately to give differently colored end-products. Where two hormones were present in the same cell, a mixed color was produced. Up to four hormones could be immunostained in a single section. Multiple immunoenzymatic staining has great potential for the analysis of plural antigen production by single cells and relationships between cells producing different antigens.

Type Journal
ISBN 0022-1554 (Print)
Authors Van Noorden, S.;Stuart, M. C.;Cheung, A.;Adams, E. F.;Polak, J. M. :
Publisher Name J HISTOCHEM CYTOCHEM
Published Date 1986-01-01 00:00:00
Published Volume 34
Published Issue 3
Published Pages 287-92
URL http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2419389
Status Published In-print