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Measurement of human phospholipase A2 in arthritis plasma using a newly developed sandwich ELISA

Abstract

A sandwich enzyme-linked immunosorbent assay (ELISA) for human non-pancreatic phospholipase A2 (PLA2) was developed using monoclonal antibodies raised against purified recombinant PLA2. This assay was shown to be specific for human non-pancreatic PLA2, showing no cross-reactivity with human pancreatic PLA2 or with snake venom PLA2 (Crotalus durissus). The immunoassay showed no cross-reactivity with plasma components, and was reproducible and quantitative between 39 pmol and 2.7 nmol PLA2/1. The levels of non-pancreatic PLA2 in the plasma of patients with arthritis was measured using this immunoassay. There were significantly higher levels of PLA2 in patients with rheumatoid arthritis than in those with osteoarthritis or healthy controls. Plasma PLA2 was highest in those patients with active rheumatoid arthritis.

Type Journal
ISBN 0263-7103 (Print)
Authors Smith, G. M.;Ward, R. L.;McGuigan, L.;Rajkovic, I. A.;Scott, K. F. :
Publisher Name Br J Rheumatol
Published Date 1992-01-01 00:00:00
Published Volume 31
Published Issue 3
Published Pages 175-8
URL http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=1540785
Status Published In-print