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Stabilized structure of the presynaptic (Y2) receptor-specific neuropeptide Y analog N-acetyl[Leu-28,Leu-31]NPY(24-36)

Abstract

Neuropeptide Y analog N-acetyl[Leu-28,Leu-31]NPY(24-36)-amide binds specifically to prejunctional or Y2 receptors acting to inhibit neurotransmitter release. The structure of this biologically active mutant was studied by two-dimensional proton nuclear magnetic resonance spectroscopy. Assignments of all backbone and side chain hydrogens were made by using totally correlated spectroscopy (TOCSY) experiments providing through-bond 1H-1H connectivities, and nuclear Overhauser effect spectroscopy (NOESY), which provided through-space and sequential backbone connectivities. Structure analysis of the peptide was performed using distance geometry and dynamic simulated annealing revealing the presence of a helical structure exhibiting an amphiphilic character and slight constriction in the segment 24-29.

Type Journal
ISBN 0006-3002 (Print)
Authors Barden, J. A.;Cuthbertson, R. M.;Potter, E. K.;Selbie, L. A.;Tseng, A. :
Publisher Name BBA-MOL BASIS DIS
Published Date 1994-01-01 00:00:00
Published Volume 1206
Published Issue 2
Published Pages 191-6
URL http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8003524
Status Published In-print