Dr Holly Holliday

My background is in gene regulation controlling cell fate in development and cancer. In 2013 I graduated with a Bachelor of Medical Science (Hons class I and the university medal), majoring in Biochemistry from the University of Sydney. During this time, I developed a strong interest in cell and mol

Biography

My background is in gene regulation controlling cell fate in development and cancer. In 2013 I graduated with a Bachelor of Medical Science (Hons class I and the university medal), majoring in Biochemistry from the University of Sydney. During this time, I developed a strong interest in cell and molecular biology.

I then undertook a PhD under the supervision of A/Prof Alex Swarbrick at the Garvan Institute of Medical Research. My thesis was focused on transcriptional regulation of stem cell maintenance and differentiation within the mammary gland by the helix-loop-helix transcription factor ID4.

I am now researching microRNA-based therapeutics for the treatment of neuroblastoma. We have performed a high-throughput screen that identified several miRNAs which could sensitise neuroblastoma cells to chemotherapy. I am now characterising the function and activity of these candidate miRNAs in cell line and animal models of neuroblastoma.

In addition, I am also working on applying new spatial transcriptomics techniques to study tumour heterogeneity and the tumour microenvironment in the context of breast cancer and melanoma.

Technical expertise and tools

  • Cell culture: primary and established cell lines, 3D organoid culture
  • Molecular cloning techniques: Restriction enzyme digest, ligation, gel electrophoresis, PCR, transformation, plasmid preps
  • Transfections: transient/stable, siRNA/miRNA/DNA vectors
  • Western blotting
  • Immunoprecipitation (IP)
  • Proximity Ligation Assay (PLA)
  • Rapid Immunoprecipitation Mass Spectrometry of Endogenous Proteins (RIME)
  • Chromatin immunoprecipitation and sequencing (ChIP-seq)
  • RNA-sequencing (RNA-seq)
  • Spatial Transcriptomics (10x Visium)
  • Basic bioinformatics analyses: Differential expression analysis, IGV, Gene set enrichment analysis (GSEA)
  • Quantitative real-time PCR (qPCR)
  • Immunocytochemistry (ICC), Immunofluorescence (IF) and Immunohistochemistry (IHC)
  • Microscopy: Fluorescence, confocal, power mosaic
  • Image analysis: QuPath, FIJI
  • Flow cytometry and fluorescence activated cell sorting (FACS)
  • Luciferase-based reporter assays
  • Proliferation, apoptosis, differentiation and colony formation assays

My background is in gene regulation controlling cell fate in development and cancer. In 2013 I graduated with a Bachelor of Medical Science (Hons class I and the university medal), majoring in Biochemistry from the University of Sydney. During this time, I developed a strong interest in cell and molecular biology.

I then undertook a PhD under the supervision of A/Prof Alex Swarbrick at the Garvan Institute of Medical Research. My thesis was focused on transcriptional regulation of stem cell maintenance and differentiation within the mammary gland by the helix-loop-helix transcription factor ID4.

I am now researching microRNA-based therapeutics for the treatment of neuroblastoma. We have performed a high-throughput screen that identified several miRNAs which could sensitise neuroblastoma cells to chemotherapy. I am now characterising the function and activity of these candidate miRNAs in cell line and animal models of neuroblastoma.

In addition, I am also working on applying new spatial transcriptomics techniques to study tumour heterogeneity and the tumour microenvironment in the context of breast cancer and melanoma.

Technical expertise and tools

  • Cell culture: primary and established cell lines, 3D organoid culture
  • Molecular cloning techniques: Restriction enzyme digest, ligation, gel electrophoresis, PCR, transformation, plasmid preps
  • Transfections: transient/stable, siRNA/miRNA/DNA vectors
  • Western blotting
  • Immunoprecipitation (IP)
  • Proximity Ligation Assay (PLA)
  • Rapid Immunoprecipitation Mass Spectrometry of Endogenous Proteins (RIME)
  • Chromatin immunoprecipitation and sequencing (ChIP-seq)
  • RNA-sequencing (RNA-seq)
  • Spatial Transcriptomics (10x Visium)
  • Basic bioinformatics analyses: Differential expression analysis, IGV, Gene set enrichment analysis (GSEA)
  • Quantitative real-time PCR (qPCR)
  • Immunocytochemistry (ICC), Immunofluorescence (IF) and Immunohistochemistry (IHC)
  • Microscopy: Fluorescence, confocal, power mosaic
  • Image analysis: QuPath, FIJI
  • Flow cytometry and fluorescence activated cell sorting (FACS)
  • Luciferase-based reporter assays
  • Proliferation, apoptosis, differentiation and colony formation assays