Amanda Brandon undertook a PhD in fetal and neonatal physiology, under the supervision of Drs Karen Gibson and Amanda Boyce at UNSW. The major component of the project was to investigate the effects of maternal renal dysfunction on the developing offspring (developmental origins hypothesis) in an attempt to uncover if their renal, cardiovascular or renin angiotensin systems had been programmed by this altered in utero environment.
Since obtaining her doctorate in 2007, Amanda has been working as a Post-Doctoral researcher at the Garvan Institute in the Diabetes and Metabolism Division under the supervision of Profs Ted Kraegen and Gregory Cooney. This is a world renowned research group in the field of diabetes, with a particular interest in the role of lipid accumulation in skeletal muscle in the development of insulin resistance and type 2 diabetes.
Amanda has used nutrient overload models (hyperglycaemia and high fat diet) to examine how excess nutrients cause insulin resistance in skeletal muscle and whether we can intervene to prevent its development. More recently she has trained in euglycaemic-hyperinsulinaemic clamp technique in mice using the dual cannulation methodology (the gold standard).
Awards and Honours
2002 – BSc (Hons Class 1), University of New South Wales - Australia
Michael J Boden*, Amanda E Brandon*, Jennifer D Tid-ang, Elaine Preston, Donna Wilks, Ella Stuart, Mark E Cleasby, Nigel Turner, Gregory J Cooney, and Edward W Kraegen. (2012) Overexpression of Manganese Superoxide Dismutase (Mn-SOD) ameliorates high fat diet induced insulin resistance in rat skeletal muscle. Am J Physiol Endocrinol Metab; 303:E798-E805 *Co-First Author
Asish K. Saha, X. Julia Xu, Thomas W. Balon, Amanda Brandon, Edward W. Kraegen and Neil B. Ruderman. (2011) Insulin resistance due to nutrient excess: Is it a consequence of AMPK downregulation? Cell Cycle 10:20, 3447-3451.
Amanda E. Brandon, Andrew J. Hoy, Nigel Turner, Bronwyn D. Hegarty, X. Julia Xu, Gregory J. Cooney, Asish K. Saha, Neil B. Ruderman, and Edward W. Kraegen. (2011) The evolution of insulin resistance in muscle of the glucose infused rat. Archives of Biochemistry and Biophysics. 509:133–141
Wright LE, Brandon AE, Hoy AJ, Forsberg GB, Lelliott CJ, Reznick J, Löfgren L, Oscarsson J, Strömstedt M, Cooney GJ, Turner N. (2011). Amelioration of lipid-induced insulin resistance in rat skeletal muscle by overexpression of Pgc-1β involves reductions in long-chain acyl-CoA levels and oxidative stress. Diabetologia. 54(6):1417-26.
Asish K. Saha, X. Julia Xu, Ebony Lawson, Rosangela Deoliveira, Amanda E. Brandon, Edward W. Kraegen, and Neil B. Ruderman. (2010) Downregulation of AMPK accompanies leucine- and glucose-induced increases in protein synthesis and insulin resistance in rat skeletal muscle. Diabetes 59:2426–2434.
Hoy, AJ, Brandon, AE, Turner, N, Watt, MJ, Bruce CR, Cooney, GJ and Kraegen, EW. (2009) Lipid and insulin infusion-induced skeletal muscle insulin resistance is likely due to metabolic feedback and not changes in IRS-1, Akt or AS160 phosphorylation. American Journal of Physiology - Endocrinology and Metabolism, 297:E67-E75.