Dr Lowenna Holt

Dr Lowenna Holt gained a BMedSc (Hons I) at the University of Sydney, majoring in Biochemistry, in 1998. She then travelled to Churchill College, University of Cambridge and undertook a PhD in the Department of Clinical Biochemistry. Her work with supervisor Prof. Ken Siddle was focused on investiga

Biography

Dr Lowenna Holt gained a BMedSc (Hons I) at the University of Sydney, majoring in Biochemistry, in 1998. She then travelled to Churchill College, University of Cambridge and undertook a PhD in the Department of Clinical Biochemistry.

Her work with supervisor Prof. Ken Siddle was focused on investigating the role of the adaptor protein Grb10 in insulin and IGF-I receptor signalling. This topic has remained the focus of her work, such that Lowenna is highly experienced in researching the role of adaptor proteins in the modulation of cellular signalling cascades.

She has worked at the Garvan Institute of Medical Research since 2003, under the guidance of Prof. Gregory Cooney and Prof. Roger Daly. Her particular interest lies in determining how these proteins modulate the size of muscle and fat tissue, and how they influence metabolic processes which ultimately impact on body composition and glucose homeostasis.

Her research findings have implications in the field of obesity and diabetes, as well as muscular disease.

Dr Lowenna Holt gained a BMedSc (Hons I) at the University of Sydney, majoring in Biochemistry, in 1998. She then travelled to Churchill College, University of Cambridge and undertook a PhD in the Department of Clinical Biochemistry.

Her work with supervisor Prof. Ken Siddle was focused on investigating the role of the adaptor protein Grb10 in insulin and IGF-I receptor signalling. This topic has remained the focus of her work, such that Lowenna is highly experienced in researching the role of adaptor proteins in the modulation of cellular signalling cascades.

She has worked at the Garvan Institute of Medical Research since 2003, under the guidance of Prof. Gregory Cooney and Prof. Roger Daly. Her particular interest lies in determining how these proteins modulate the size of muscle and fat tissue, and how they influence metabolic processes which ultimately impact on body composition and glucose homeostasis.

Her research findings have implications in the field of obesity and diabetes, as well as muscular disease.

Awards and Honours

2012 - 2nd prize, Micrograph of the Year Competition, Bosch Institute for Medical Research, University of Sydney
2011 - Award for best postdoctoral oral presentation at the NSW Cell and Developmental Biology Meeting
Australia and New Zealand Society for Cell and Developmental Biology
2006 - ASMR Medal for best oral presentation at the ASMR Scientific Meeting Australian Society for Medical Research
2002 - Sackler Studentship for Medical Sciences School of Clinical Medicine, University of Cambridge

Education

2003 - PhD, University of Cambridge - UK
1998 - BMedSc (Hons I), University of Sydney - Australia

Selected Publications

Mokbel N, Hoffman NJ, Girgis CM, Small L, Turner N, Daly RJ, Cooney GJ, Holt LJ. Grb10 Deletion Enhances Muscle Cell Proliferation, Differentiation and GLUT4 Plasma Membrane Translocation. J Cell Physiol. 2014 Mar 24.

Holt LJ, Turner N, Mokbel N, Trefely S, Kanzleiter T, Kaplan W, Ormandy CJ, Daly RJ, Cooney GJ. Grb10 regulates the development of fiber number in skeletal muscle. FASEB J. 2012 Sep;26(9):3658-69.

Berg JS, Lin KK, Sonnet C, Boles NC, Weksberg DC, Nguyen H, Holt LJ, Rickwood D, Daly RJ, Goodell MA. Imprinted genes that regulate early mammalian growth are coexpressed in somatic stem cells. PLoS One. 2011;6(10):e26410.

Holt LJ, Lyons RJ, Ryan AS, Beale SM, Ward A, Cooney GJ, Daly RJ. Dual ablation of Grb10 and Grb14 in mice reveals their combined role in regulation of insulin signaling and glucose homeostasis. Mol Endocrinol. 2009 Sep;23(9):1406-14.

Rajala A, Daly RJ, Tanito M, Allen DT, Holt LJ, Lobanova ES, Arshavsky VY, Rajala RV. Growth factor receptor-bound protein 14 undergoes light-dependent intracellular translocation in rod photoreceptors: functional role in retinal insulin receptor activation. Biochemistry. 2009 Jun 23;48(24):5563-72.

Goenaga D, Hampe C, Carré N, Cailliau K, Browaeys-Poly E, Perdereau D, Holt LJ, Daly RJ, Girard J, Broutin I, Issad T, Burnol AF. Molecular determinants of Grb14-mediated inhibition of insulin signaling. Mol Endocrinol. 2009 Jul;23(7):1043-51.

Smith FM, Holt LJ (Joint 1st Authors), Garfield AS, Charalambous M, Koumanov F, Perry M, BazzaniR, Sheardown SA, Hegarty BD, Lyons RJ, Cooney GJ, Daly RJ, Ward A. Mice with a disruption of the imprinted Grb10 gene exhibit altered body composition, glucose homeostasis, and insulin signaling during postnatal life. Mol Cell Biol. 2007 Aug;27(16):5871-86. Epub 2007 Jun 11.

Depetris RS, Hu J, Gimpelevich I, Holt LJ, Daly RJ, Hubbard SR. Structural basis for inhibition of the insulin receptor by the adaptor protein Grb14. Mol Cell. 2005 Oct 28;20(2):325-33.

Holt LJ, Daly RJ. Adapter protein connections: the MRL and Grb7 protein families. Growth Factors. 2005 Sep;23(3):193-201. Review.

Holt LJ, Siddle K. Grb10 and Grb14: enigmatic regulators of insulin action--and more? Biochem J. 2005 Jun 1;388(Pt 2):393-406. Review.

Conigrave AD, van der Weyden L, Holt L, Jiang L, Wilson P, Christopherson RI, Morris MB. Extracellular ATP-dependent suppression of proliferation and induction of differentiation of human HL-60 leukemia cells by distinct mechanisms. Biochem Pharmacol. 2000 Dec 1;60(11):1585-91.

Elaissari A, Holt L, Meunier F, Voisset C, Pichot C, Mandrand B, Mabilat C.  Hydrophilic and cationic latex particles for the specific extraction of nucleic acids. J Biomater Sci Polym Ed. (1999). 10:403-20.