Dr Max Nobis

Research Officer

Following my MSc in Oncology at the Vrije Universiteit in Amsterdam, I went on to work in Prof Kurt Anderson’s lab at the CRUK Beatson Institute in Glasgow. My PhD project focused on characterising FRET biosensor mice and their use in studying disease progression in mouse models of mammary and pancreatic cancer, as well as their use in monitoring pharmacodynamics in vivo, employing optical window imaging.

I joined Dr Paul Timpson’s lab to apply advanced imaging techniques in the study of pancreatic cancer disease progression and treatment, using FRET biosensor mice in conjunction with genetic mouse models of pancreatic cancer and patient specific biomarkers for the tailoring of personalized treatment regimens.

Research Interests

Advanced Imaging Techniques
Fluorescent Biosensors
Invasion and Metastasis
Signal Transduction in Cancer
Pancreatic Ductal Adenocarcinoma
Tumour Microenvironment
Personalised Medicine

Education

2015 – PhD, CRUK Beatson Institute, University of Glasgow – UK
2012 – MSc, Vrije Universiteit Amsterdam – Netherlands
2009 – BSc, University of Applied Sciences Bonn-Rhein-Sieg – Germany

Selected Publications

WildenbergME, Koelink PJ, Diederen K, te Velde AA, Wolfkamp SC, Nuij VJ, Peppelenbosch MP, Nobis M, Sansom OJ, Anderson KI, van der Woude CJ, D’Haens GR, and van den Brink GR. The ATG16L1 risk allele associated with Crohn's disease results in a Rac1-dependent defect in dendritic cell migration that is corrected by thiopurines. Mucosal Immunol. 2016 Jul 20 

Kalafatovic D*, Nobis M*, Son J, Anderson KI, Ulijn RV. MMP-9 triggered self-assembly of doxorubicin nanofiber depots halts tumor growth. Biomaterials. 2016 Aug; 98:192-202 *Co-first author

Susanto O, Muinonen-Martin AJ, Nobis M, Insall RH. Visualizing cancer cell chemotaxis and invasion in 2D and 3D. Methods Mol Biol. 2016; 1407:217-28

Erami Z*, Herrmann D*, Warren SC, Nobis M, McGhee EJ, Lucas M, Leung W, Reischmann N, Mrowinska A, Schwarz JP, Kadir S, Conway JRW, Vennin C, Karim SA, Campbell AD,  Gallego-Ortega D,  Magenau A, Murphy KJ, Ridgway RA,  Law AM,  Walters SN, Grey ST, Croucher D,  Zhang L, Herzog H, Hardeman EC, Gunning PW, Ormandy CJ, Evans JTR,  Strathdee D,  Sansom OJ, Morton JP1, Anderson KI, Timpson P. Intravital FRAP imaging using an E-cadherin-GFP mouse reveals disease and drug-dependent dynamic regulation of cell-cell junctions in live tissue. Cell Reports. 2016 Jan 5;14(1):152-67

von Karstedt S, Conti A, Nobis M, Montinaro A, Hartwig T, Lemke J, Legler K, Annewanter F, Campbell AD, Taraborrelli L, Grosse-Wilde A, Coy JF, El-Bahrawy MA, Bergmann F, Koschny R, Werner J, Ganten TM, Schweiger T, Hoetzenecker K, Kenessey I, Hegedüs B, Bergmann M, Hauser C, Egberts JH, Becker T, Röcken C, Kalthoff H, Trauzold A, Anderson KI, Sansom OJ, Walczak H. Cancer cell-autonomous TRAIL-R signaling promotes KRAS-driven cancer progression, invasion, and metastasis. Cancer Cell. 2015 Apr 13;27(4):561-73 

Kalafatovic D, Nobis M, Javid N, Frederix PW, Anderson KI, Saunders BR, Ulijn RV. MMP-9 triggered micelle-to-fibre transitions for slow release of doxorubicin. Biomater Sci. 2015 Feb;3(2):246-9

Nobis M, McGhee EJ, Herrmann D, Magenau A, Morton JP, Anderson KI, Timpson P. Monitoring the dynamics of Src activity in response to anti-invasive dasatinib treatment at a subcellular level using dual intravital imaging. Cell Adhesion & Migration. 2014 Jul ;8(5):478-86

Lo Re D, Zhou Y, Nobis M, Anderson KI, Murphy PV. Synthesis of migrastatin and its macroketone analogue and in vivo FRAP analysis of the macroketone on E-cadherin dynamics. Chembiochem. 2014 Jul 7;15(10):1459-64. 

Johnsson AK*, Dai Y*, Nobis M, Baker MJ, McGhee EJ, Walker S, Schwarz JP, Kadir S, Morton JP, Myant KB, Huels DJ, Segonds-Pichon A, Sansom OJ, Anderson KI, Timpson P, Welch HC. The Rac-FRET mouse reveals tight spatiotemporal control of Rac activity in primary cells and tissues. Cell Reports. 2014 Mar 27;6(6):1153-64 

Nobis M, Carragher NO, McGhee EJ, Morton JP, Sansom OJ, Anderson KI, Timpson P. Advanced intravital subcellular imaging reveals vital three-dimensional signalling events driving cancer cell behaviour and drug responses in live tissue. FEBS Journal. 2013 Nov;280(21):5177-97 

Nobis M, McGhee EJ, Morton JP, Schwarz JP, Karim SA, Quinn J, Edward M, Campbell AD, McGarry LC, Evans TR, Brunton VG, Frame MC, Carragher NO, Wang Y, Sansom OJ, Timpson P, Anderson KI. Intravital FLIM-FRET imaging reveals dasatinib-induced spatial control of src in pancreatic cancer. Cancer Research. 2013 Aug 1;73(15):4674-86

Trinidad AG, Muller PA, Cuellar J, Klejnot M, Nobis M, Valpuesta JM, Vousden KH. Interaction of p53 with the CCT complex promotes protein folding and wild-type p53 activity. Moleluar Cell. 2013 Jun 27;50(6):805-17

Timpson P, McGhee EJ, Erami Z, Nobis M, Quinn JA, Edward M, Anderson KI. Organotypic collagen I assay: a malleable platform to assess cell behaviour in a 3-dimensional context. Journal of Visual Experiments. 2011 Oct 13;(56):e3089

Dr Max Nobis