DNA / RNA Extraction at Garvan Molecular Genetics
High and low throughput Nucleic Acid Extraction
Garvan Molecular Genetics provides a nucleic acid extraction service in a NATA ISO 17025 and ISO 15189 quality controlled environment. The DNA or RNA extraction is extracted with spin columns and has, therefore, the highest possible quality available. Our average extraction performance is shown in the table below:
|Concentration (depending on primary sample material)||5 - 10ug|
|Ratio 260/280||1.9 - 2.3|
|Ratio 260/230||1.8 - 2.2|
|Elution volume||30 - 200ul|
|RIN value for RNA samples||8 - 10|
We accept the following primary sample material:
-RNA from blood via PAXgene tubes (RNA can be extracted from EDTA blood tubes but for gene expression comparison the use of PAXGene tubes is highly recommended)
-RNA from soft tissue (no homogenization needed, samples will be frozen in liquid nitrogen and pulverized before lysis)
-RNA from hard tissue (homogenization via tissue disruptor and lysis beads)
-RNA from cell culture or sorted cells (min 104 to max 107)
-micro RNA and mitochondrial RNA upon request isolated with specialized kits
-RNA from FFPE samples
-DNA from blood, buffy coat, semen
-DNA from soft or hard tissue
-DNA from cell culture or sorted cells (min 104 to max 107)
-DNA from saliva via OraCollect swabs
-DNA from serum (free floating DNA) with a specialized kit
-DNA from urine with a specialized kit
-DNA from mouse feces with a specialized kit
-DNA from FFPE samples
There are separate modules for extraction of samples in single tubes and submission of samples in 96x well plates.
For all samples that require additional preparative steps before extraction (for example FFPE, Serum, Urine, Paxgene tubes or difficult tissue samples that require homogenization) we offer clients to perform the preparative steps themselves and reduce the cost of extraction.
There are three levels of turnaround time for this service for standard samples (without further preparation steps):
- normal (4 working days, standard price)
- urgent (48h standard price +50% surcharge)
- very urgent (24h standard price +100% surcharge)
For prices please see our Molecular Genetics Shop webpage.
Please follow our sample submission guidelines (see above) and use our online sample submission portal to submit your samples (Access to our Online Sample Submission Portal) and send samples via Australia mail or courier to:
Attn. Nicola Tait
Garvan Molecular Genetics
Garvan Institute of Medical Research
384 Victoria Street
Darlinghurst, NSW, 2010
(Internal clients can drop off RNA samples in freezer 8457 and DNA samples in fridge 5117 in the corridor to TKCC, Garvan level 8 in front of room 8.05)
Extracted Sample Reception
Once your samples have been processed and the DNA or RNA samples are ready for collection you will be notified by our online sample submission software via email to your nominated email address. Samples can be picked up or sent via courier at your cost.
Workflow for Qiagen Spin Column Extraction
The actual process of DNA extraction follows the usual workflow of column-based DNA cleanups. Binding of DNA in high salt conditions to silica membranes, washing with Ethanol based wash-buffers and elution in low salt conditions.
The DNA is extracted in a buffer which contains some Tris, no EDTA and is adjusted to a pH of 8.
You can choose the elution volume to between 30ul and 200ul.