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DNA / RNA Extraction


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Online Sample Submission Portal Instructions

Sample Submission Guidelines

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How to submit samples online video 

How to download results video

 




DNA / RNA Extraction at Garvan Molecular Genetics

 

Spin Columns versus Magnetic Bead Extraction

Garvan Molecular Genetics is now providing a nucleic acid extraction service. The DNA or RNA extraction with spin columns will be performed manually (single spin columns) for precious samples or with a high throughput automated procedure (96x well spin column plates) for animal tissue or blood samples. Alternatively, we offer a DNA or RNA extraction with magnetic beads on various robotic platforms (QiaSymphony/ Zephyr). We extract from tissue, cells, serum, swabs, FFPE, blood and buffy coat samples. The extraction volume can be chosen according to the needs of the post-extraction processes.

For all samples that require additional preparative steps before extraction (for example FFPE, Serum, Urine, Paxgene tubes or difficult tissue samples that require homogenization) we offer clients to perform the preparative steps themselves and reduce the cost of extraction.

 qiacubehtzephyr

 

Turnaround time 

There are three levels of turnaround time for this service for standard samples (without further preparation steps):

  • normal (48h, standard price)
  • urgent (24h standard price +50% surcharge)
  • very urgent (8h standard price +100% surcharge)

 

Pricing

For prices please see our Molecular Genetics Shop webpage.

 

Sample Submission


Please follow our sample submission guidelines (see above) and use our online sample submission portal to submit your samples (Access to our Online Sample Submission Portal) and send samples via Australia mail or courier to: 

Attn. Teighan Greaves

Garvan Molecular Genetics

Garvan Institute of Medical Research

384 Victoria Street

Darlinghurst, NSW, 2010

(Internal clients can drop off RNA samples in freezer 8457 and DNA samples in fridge 5117 in the corridor to TKCC, Garvan level 8 in front of room 8.05)

 

Extracted Sample Reception

 

Once your samples have been processed and the DNA or RNA samples are ready for collection you will be notified by our online sample submission software via email to your nominated email address. Samples can be picked up or sent via courier at your cost. 

 

DNA easy kit

 

DNA Extraction Workflow

Workflow for Qiagen Spin Column Extraction

The actual process of DNA extraction follows the usual workflow of column-based DNA cleanups. Binding of DNA in high salt conditions to silica membranes, washing with Ethanol based wash-buffers and elution in low salt conditions.

The DNA is extracted in a buffer which contains some Tris, no EDTA and is adjusted to a pH of 8.

You can choose the elution volume to between 30ul and 200ul.

 

 

Workflow for Magnetic Bead Extraction

Binding of DNA occurs to the magnetic particles in high salt conditions, washing with Ethanol based wash-buffers and elution in low salt conditions.

The DNA is extracted in a buffer which contains some Tris, no EDTA and is adjusted to a pH of 8.

You can choose the elution volume to between 10ul and 300ul.

 magnetic

bottle