Documents and Links

Documents

Please see the service webpages for Sample Submission Forms and Sample Submission Guidelines 

DNA Extraction Service

Mouse Genotyping Service 

Sequencing Service

SNP Genotyping Service

Reagent Ordering Service

Oligo Order Service

Cell line Identification Service

Agilent Bionalyser

• GMG Bioanalyzer User SOP (Word)
• Agilent trouble shooting guide (PDF)
• Bioanalyser user manual (PDF)
• RNA Nano Series 2 Kit Guide (PDF)
• RNA Pico 6000 Kit Guide (PDF)
• DNA 1000 Kit Guide (PDF)
• DNA High Sensitivity Kit Guide (PDF)

BioRad Geldoc System and Ethidiumbromide substitution

• GMG Geldoc SOP (Word)
• SWP Geldoc - Taking a gel image (Word)
• Usage of Gelred (Word)
• GelRed Flyer (PDF)
• Usage of Diamond Nucleic Acid Dye (PDF) 
• The Fusion or Ebox software analyzes any gel photo and is available under \\Pandora\Volumes\Software\Windows\GMG_Software\Fusion Fx7 or \\Pandora\Volumes\Software\Windows\GMG_Software\E-Box

BioRad CFX1000 Realtime instrument

• Biorad CFX1000 Manual
• Analysis software for BioradC1000 under \\Pandora\Volumes\Software\Windows\GMG_Software\Biorad CFX1000

EPmotion Robots

• GMG EPmotion installation SOP (Word)
• GMG EPmotion programming SOP (Word)
• EPmotion 5070 manual (PDF)

Methylation Quantification

• Bisulphite QC NucAcidRes (PDF)
• Cytosine methylation of cancer cell lines Ehrich PNAS (PDF)
• EpiSymposium (PDF)
• Introduction to DNA Methylation using the MassARRAY system (PDF)
• Sequenom Standard EpiPanel (PDF)
• Prof Susan Clark: Genomic profiling of CpG methylation and allelic specificity using quantitative high-throughput mass spectrometry: critical valuation and improvements (PDF)

Nanodrop

• Nanodrop Operation Manual (PDF)

Next Generation Sequencing Protocols

• RNA Seq Protocol (PDF)

Ordering through the Garvan Molecular Genetics Shop

• Reagent Order Form (Excel)

Qbit DNA + RNA Quantification

• GMG Qbit Operation SOP (Word)
• Qubit Operation Manual (PDF)

Realtime PCR Information

• The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments (PDF)
• Endogenous Control Selection (PDF)
• Latest Guidelines for Performing Realtime PCR (for Experts) (PDF)
• Quant PCR on the 7900 (PDF)
• Realtime Bible (dont get bored, read from page 35 to 50) (PDF)
• Realtime PCR Theory (PDF)
• Realtime PCR with ABI chemistry (PDF)
• Workshop Slides: Basics of Realtime PCR (PDF)
• How to find a (non) inventoried Taqman probe - Online Ordering Guide for TaqMan Gene Expression Assays (PDF)

3D digital PCR instrument

• Quantstudio 3D digital PCR User Manual
• Next Generation Sequencing (Illumina) library quantification on Quantstudio 3D
• Next Generation Sequencing conversion Excel file
• Copy Number Variance Analysis
• SYBRgreen Assay Analysis
• Analysis software for Quantstudio 3D is cloud based

Sequencing

• FinchTV for viewing your sequencing results (ZIP folder with exe File)
• SeqScanner 2 Software for viewing Sequencing Results (link)
• Difficult Template (PDF)
• GeneScan 600 Size Standard Sizes (PDF)
• GeneScan 500 Size Standard Sizes (JPG)
• Sequence Enhancer Information (Word)
• Sequencing Workshop (PDF)
• Sonification of Plasmid (PDF)
• Templiphi Method (PDF)

Genescanning program for viewing results

• Peak Scanner Software v1.0 (.exe File)
• SeqScanner 2 Software (link)

SNP Genotyping

• GMG Sequenom Iplex gold SNP detection SOP (Word)
• Sequenom iPLEX Assay (PDF)

UPL Probe and SYBR Mastermix Setup protocols

• UPL Probe Setup (Word)
• SensidT SYBR Setup (Word)

 Licor Odyssey

• Good Western Blot gone bad (PDF)
• Western Blot Infrared Dyes  (PDF)
• Western Blots on the Licor Quick Setup Chart (PDF)
• Analysis software under \\Pandora\Volumes\Software\Windows\GMG_Software\LICOR ODYSSEY

Vilber Lourmat Fusion FX7 and E-Box

• Fusion FX7 product description (PDF)
• Fusion Manual 2016 (PDF)
• Analysis software for Fusion Fx7 under \\Pandora\Volumes\Software\Windows\GMG_Software\Fusion Fx7
• Analysis software for E-Box under \\Pandora\Volumes\Software\Windows\GMG_Software\E-Box
• Precision Plus Protein WesternC standards (without StrepTactin-HRP conjugate), cat. #161-0376,

http://www.bio-rad.com/en-au/sku/161-0376-precision-plus-protein-westernc-standards?parentCategoryGUID=5b12619f-229a-4b3b-a41e-11141912fec8

• Precision Plus Protein WesternC Pack (with StrepTactin-HRP conjugate), cat. # 161-0385

http://www.bio-rad.com/en-au/sku/161-0385-precision-plus-protein-westernc-pack?parentCategoryGUID=1d045ed2-e2d3-4c21-9d1c-a956dbaf6b21

ClarioStar BMG

• ClarioStar MARS Data Analysis Software Operation Manual (PDF)
• Analysis software under \\Pandora\Volumes\Software\Windows\GMG_Software\ClarioStar

Links

• Applied Biosystems / Invitrogen / Lifetechnologies (Taqman probes): www.appliedbiosystems.com.au
• UPL probes design: www.universalprobelibrary.com
• Sequenom (now rebranded as Agena Bioscience): mysequenom.com
• Primers from IDT: www.idtdna.com
• Primers from Sigma: www.sigma-aldrich.com
• DNA / RNA / PCR cleanup kits from Qiagen: www.qiagen.com
• Mouse genome informatics: www.informatics.jax.org
• NCBI: www.ncbi.nlm.nih.gov
• SeqScanner 2: http://resource.thermofisher.com/page/WE28396_2/
• Roche: www.roche.com
• Bioanalyzer 2100: www.agilent.com.au
• BioRad: www.bio-rad.com
• Australian BioResources Ltd (Garvan-owned research mice breeding and holding facility): www.abr.org.au