Documents and Links
Documents
Please see the service webpages for Sample Submission Forms and Sample Submission Guidelines
Cell line Identification Service
BioRad Geldoc System and Ethidiumbromide substitution
- GMG Geldoc SOP (Word)
- SWP Geldoc - Taking a gel image (Word)
- Usage of Gelred (Word)
- GelRed Flyer (PDF)
- Usage of Diamond Nucleic Acid Dye (PDF)
- The Fusion or Ebox software analyzes any gel photo and is available under \\Pandora\Volumes\Software\Windows\GMG_Software\Fusion Fx7 or \\Pandora\Volumes\Software\Windows\GMG_Software\E-Box
BioRad CFX1000 Realtime instrument
- Biorad CFX1000 Manual
- Analysis software for BioradC1000 under \\Pandora\Volumes\Software\Windows\GMG_Software\Biorad CFX1000
EPmotion Robots
- GMG EPmotion installation SOP (Word)
- GMG EPmotion programming SOP (Word)
- EPmotion 5070 manual (PDF)
LightCycler 480 Realtime instrument
- LightCycler 480 Operation Manual (PDF)
- LightCycler 480 Software SOP (PDF)
- Analysis software for LC480 under \\Pandora\Volumes\Software\Windows\GMG_Software\LightCycler 480
Nanodrop
Ordering through the Garvan Molecular Genetics Shop
- Reagent Order Form (Excel)
Qbit DNA + RNA Quantification
- GMG Qbit Operation SOP (Word)
- Qubit Operation Manual (PDF)
Realtime PCR Information
- The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments (PDF)
- Endogenous Control Selection (PDF)
- Latest Guidelines for Performing Realtime PCR (for Experts) (PDF)
- Quant PCR on the 7900 (PDF)
- Realtime Bible (dont get bored, read from page 35 to 50) (PDF)
- Realtime PCR Theory (PDF)
- Realtime PCR with ABI chemistry (PDF)
- Workshop Slides: Basics of Realtime PCR (PDF)
- How to find a (non) inventoried Taqman probe - Online Ordering Guide for TaqMan Gene Expression Assays (PDF)
7900 Realtime Instrument
- 7900 Operation Manual (PDF)
- RQ Manager Software Manual (PDF)
- Analysis software for 7900 under \\Pandora\Volumes\Software\Windows\GMG_Software\SDS 2.3 revA Installer.exe
Quantstudio 7 Realtime instrument
- Quantstudio7 Quick Setup Guide
- Quantstudio7 High Resolution Meltcurve Module
- Quantstudio 7 Gene Expression Module
- Analysis software for Quantstudio 7 is called Expression Suite (click for link to webpage to download)
- ExpressionSuite Analysis tutorial
3D digital PCR instrument
- Quantstudio 3D digital PCR User Manual
- Next Generation Sequencing (Illumina) library quantification on Quantstudio 3D
- Next Generation Sequencing conversion Excel file
- Copy Number Variance Analysis
- SYBRgreen Assay Analysis
- Analysis software for Quantstudio 3D is cloud based
Sequencing
- FinchTV for viewing your sequencing results (ZIP folder with exe File)
- SeqScanner 2 Software for viewing Sequencing Results (link)
- Difficult Template (PDF)
- GeneScan 600 Size Standard Sizes (PDF)
- GeneScan 500 Size Standard Sizes (JPG)
- Sequence Enhancer Information (Word)
- Sequencing Workshop (PDF)
- Sonification of Plasmid (PDF)
- Templiphi Method (PDF)
Genescanning program for viewing results
UPL Probe and SYBR Mastermix Setup protocols
- UPL Probe Setup (Word)
- SensidT SYBR Setup (Word)
Links
- Applied Biosystems / Invitrogen / Lifetechnologies (Taqman probes): www.appliedbiosystems.com.au
- UPL probes design: www.universalprobelibrary.com
- Sequenom (now rebranded as Agena Bioscience): www.agenacx.com
- Primers from IDT: www.idtdna.com
- Primers from Sigma: www.sigma-aldrich.com
- DNA / RNA / PCR cleanup kits from Qiagen: www.qiagen.com
- Mouse genome informatics: www.informatics.jax.org
- NCBI: www.ncbi.nlm.nih.gov
- SeqScanner 2: http://resource.thermofisher.com/page/WE28396_2/
- Roche: www.roche.com
- Bioanalyzer 2100: www.agilent.com.au
- BioRad: www.bio-rad.com
- Australian BioResources Ltd (Garvan-owned research mice breeding and holding facility): www.abr.org.au
