Molecular Genetics Prices

 

As a Garvan researcher you have access to a special discounted price (please see here). If you work on the precinct you will pay a precinct price. As an external entity to Garvan the below prices are applicable to your cost calculation. We also have different prices for commercial clients, please enquire at CustomerService@garvan.org.au.

 

Reagent Ordering Form

   

We buy in bulk and get special prices for you.

When we provide you with reagents you profit from bulk pricing and you do not have to pay for delivery costs.

Our reagents are available 24/7 as you can send an email to and let us know what you have used when you work on weekends or after hours. Please do remember to send us this email as this system is based on trust. Alternatively leave a short message on the desk of the Sequencing Service Coordinator with your full name and the reagent or consumable you have taken.

 

Mouse Genotyping Service Prices 

All PCRs are run with internal control housekeeping PCR and a WT control DNA additional to gene specific controls (if provided), these controls are included in the price listed. Urgent samples are charged at double the listed price.

Mouse Genotyping 1 PCR $5.95 Shop Meltcurve
Mouse Genotyping 2 PCRs $7.90
Mouse Genotyping 3 PCRs $10.12
HRM SNP detection (any SNP also human) $7.59
Copy Number Het/Hom Differentiation. This assay has a risk of false results, this risk can be minimised by submitting a het and a hom control with the samples, (control DNA prepped with the samples). The DNA quality is crucial for this test, we require 50ul of DNA at 50ng/ul (all DNAs diluted to this same concentration). The results of this assay cannot be translated directly in a breeding scheme but must be verified by cross-testing breeding strategy.  $75  
Project establishment for a new PCR (primer design, ordering of primers, testing of PCR with known controls or DNA of animal tissue with known genotypes) $120
Project establishment research level for a new PCR (primer design, ordering of primers, testing of PCR with different TAQenzymes, redesign if necessary until PCR is successfully established) $350
DNA extraction from mouse tissue for genotyping $2.76
 Pathogen Testing
Helicobacter ssp. ((bilis, muridarum, hepaticus, typhlonius, rodentium)) $19.84
Pasteurella ssp (Javetz, Heyl) $19.84
Corynebacterium bovis $19.84
DNA extraction from tissue $2.76
DNA extraction from swab $4.14
DNA extraction from fecal sample $11.55

 

 

Cell line Identification Service Prices 

Human Cell lines (for DNA samples)

$165  Shop Cell Line

Human Cell lines (for cell pellets)

$173.50  

Mouse  Cell lines (for DNA samples)

$165.00  

Mouse Cell lines (for cell pellets)

$173.50

 

We perform a Mycoplasma test via PCR. We amplify in regions of the 23s and the 16s rRNA and find the follwing species:

Mycoplasma hyorhinis, orale, hominis,  argininis, salivarium,  pirum, fermentans,   and Acholeplasma laidlawii, account for over 95% of isolates from cultured  cells. In addition we are able to detect Mycoplasma pneumoniae, gallisepticum, genitalium,   penetrans, synoviae, bovis, hyopneumoniae,Ureaplasma urealyticum, Spiroplasma citri.

$64.20 Mycoplasma picture

 

 Sequencing & Fragment Separation Service 

Service Sample Price Shop Sequencing

Premix primer and PCR or Plasmid 

(you provide the plasmid DNA or PCR product at the right concentration premixed with the primer at the right concentration [please see on our sequencing page for further details]. We run Sanger sequencing and the results will be uploaded to our portal).

 

$5.50

Fragment Separation without size standard

$4.20
Fragment Separation with size standard $5.70

cDNA transcription reaction per sample

$15.12

Agarose Gel Run

10.60

Plasmid Mix & SEQ

(you provide the plasmid DNA, we measure the DNA concentration, dilute to optimal conc. and mix with the sequencing primer and run Sanger sequencing, the results will be uploaded to our portal).

$17.28

PCR Mix & SEQ (bidirectional)

(you provide the PCR product, we cleanup the PCR product, we measure the DNA concentration, dilute to optimal conc. and mix with the sequencing primer and run Sanger sequencing, the results will be uploaded to our portal).

$26.33

PCR Setup & SEQ (bidirectional) without analysis

(you provide the DNA and the primer sequence for the PCR, we setup & cleanup the PCR product, we measure the DNA concentration, dilute to optimal conc. and mix with the sequencing primer and run Sanger sequencing)

We will send you the abi.files and a text file that you can analyze yourself 

$60.75

PCR Setup & SEQ (bidirectional) with analysis

(you provide the DNA and the primer sequence for the PCR, we setup & cleanup the PCR product, we measure the DNA concentration, dilute to optimal conc. and mix with the sequencing primer and run Sanger sequencing)

We align the results against the reference sequence and send you an alignment screenshot (no report).

$108

PCR Design & SEQ (bidirectional) with analysis and report

(you send us information about the mutation you are interested in and the associated reference sequence and we design primers,  we do the PCR setup, PCR cleanup, dilution of PCR product, SEQ setup and trace QC )

We align the results against the reference sequence and generate a report detailing the mutation(s) found.

$202.50

 

Clinical Diagnostic Sanger Sequencing & Copy Number Analysis

Clinical Diagnostic Sanger Sequencing (confirmatory) with technical report (ISO 17025) $310.50
Large Scale Clinical Diagnostic Sequencing Projects  upon request

 

 

  DNA/RNA Extraction Service 

Sample Type Material Price  
     

DNA High throughput

(>96 samples)

Whole Blood or Buffy Coat (200 ul in 96 well plate)  ~4 to 5ug total DNA yield                                          $12.83

DNA High throughput

(>96 samples)

Tissue  or Cells (already precut to 20 to 40mg pr 5 to 10 x 106 cells in 96 well plate) without homogenisation   ~4 to 5ug total DNA yield        $11.48

DNA Manual

Whole Blood or Buffy Coat (200 ul)  ~4 to 5ug total DNA yield                                             $13.50

DNA Manual

Cells or Fresh Frozen Tissue (already precut to 20 to 40mg pr 5 to 10 x 106 cells) without homogenisation     ~4 to 5ug total DNA yield        $14.18
DNA Manual hard Tissue (incl. homogenization)  $43.88
DNA Manual Buccal Swab (OraCollect) $48.33
DNA Manual FFPE (total of 20um cuts)  $50.63 
DNA Manual DNA isolation from Urine or Serum (1 to 5ml) via Qiamp kit via concentration with the same spin column $28.35
DNA Manual DNA midi kit isolation from 1ml of blood $51.98
DNA Manual DNA isolation from Stool or Soil (with special fecal DNA extraction kit) non human $45.90
DNA Manual DNA and mRNA extraction from 5ml Urine or Serum via the QIAamp Circulating Nucleic Acid Kit with vacuum $126.23



RNA High throughput

(>96 samples)

Whole Blood or Buffy Coat (200 ul in 96 well plate)  6-8 ug total RNA yield $24.30

RNA High throughput

(>96 samples)

Cells or Fresh Frozen Tissue (already precut to 20 to 40mg pr 5 to 10 x 106 cells in 96 well plate) without homogenisation    6-8 ug total RNA yield $22.95

RNA Manual

 Whole Blood or Buffy Coat (200 ul)   6-8 ug total RNA $31.05

RNA Manual

Cells or Fresh Frozen Tissue (excl.homogenization)       2-3 ug total RNA yield    $29.70
RNA Manual FFPE (extracted RNA is DNAse treated) $56.03 
RNA Manual hard Tissue (incl. homogenization) $66.83 
RNA Manual PAXgene tube (tube provided by client) (extracted RNA is DNAse treated) $61.02   
RNA Manual DNA and mRNA extraction from 5ml Urine or Serum via the QIAamp Circulating Nucleic Acid Kit with vacuum $126.23
RNA Manual miRNA from blood, cells and tissue $55.62
DNA/RNA Quality Check (QC) Nanodrop $2.43
DNA/RNA Quality Check (QC) QBit $3.24
     
DNA TapeStation 4200 quality check of genomic DNA/sample $11.88
RNA Tapestation 4200 quality check of mRNA/sample $11.88
RNA DV200 determination for FFPE samples $15.93  

 

  Primer Ordering Service

Sigma-Aldrich Oligos

Primer Ordering Service
Standard desalted Oligo (25 nmol scale) length 15- 60 nucleotides (delivery 4-5 working days)

$7.00 

no express service avilable anymore  
HPLC, PAGE purification (minimum scale is 25nmol) $35.00
Ultramere length 60- 120 nucleotides (4nmol) $1.50/nt
Modified Oligos, 5' or 3' Phos, Biotin, FAM, VIC, etc (100 nmol scale minimum) price on request

IDT

Standard desalted Oligo (25 nmol scale) length 15-34 nucleotides (delivery ~3 working days) $7.00 
Standard desalted Oligo (25 nmol scale) length 10-59 nucleotides $10.00 
Standard desalted Oligo (100 nmol scale) length 10-90 nucleotides $26.00
HPLC, PAGE purification (minimum scale is 100nmol) $45.00
Modified Oligos, 5' or 3' Phos, Biotin, FAM, VIC, etc (100 nmol scale minimum) price on request

Ultramers from IDT

Ultramer 4nmole (65 - 200 Bp) price per Base $ 1.10
Ultramer 20 nmole (65 - 200 Bp) price per Base $ 2.15
PAGE Ultramer (45 -200Bp) price per Base $ 1.10

gBlocks from IDT

gBlocks® Gene Fragments, 125-500 bp $ 105.00
gBlocks® Gene Fragments, 501-750 bp $ 145.00
gBlocks® Gene Fragments, 751-1000 bp $ 165.00
gBlocks® Gene Fragments, 1001-1250 bp $ 225.00
gBlocks® Gene Fragments, 1251-1500 bp $ 265.00
gBlocks® Gene Fragments, 1501-1750 bp $ 305.00
gBlocks® Gene Fragments, 1751-2000 bp $ 345.00

  

  Microtiter Plates

ABI plates 10X $115.00 Microtiter Plates
BioRad 96 well plates 5X $30.00
Roche LC480 plates 10X $115.00
Optical seals 10X $35.00

 

  Master Mixes

Taqman Power SYBR Mastermix 5ml $350.00 Master Mixes
Taqman Gene Expression Mastermix 5ml $350.00

 

  Lifetechnology (formerly ABI) Assays

ABI Taqman Assay (inventoried) XS

100x 10ul reactions (50ul, use 0.5ul)

$100.00 Lifetechnology (formerly ABI) Assays

ABI Taqman Assay (inventoried) S

500x 10ul reactions (250ul, use 0.5ul)

$269.00

ABI Taqman Assay (inventoried) M

750x 10ul reactions (375ul, use 0.5ul)

$950.00

ABI Custom Made (non-inventoried)  MED

750x 10ul reactions (375ul, use 0.5ul)

$1040.00

ABI Custom Made (non-inventoried)  SMALL

500 x 10ul reactions (250ul, use 0.5ul)

$590.00
TaqMan MGB probes (6,000 pmoles) $710.00
TaqMan® SNP Genotyping Assay, Human SMALL $500.00
TaqMan® SNP Genotyping Assay, Human MED $1000.00
TaqMan® miRNA Assays (inventoried) $294.00
TaqMan® miRNA Assays (non-inventoried) $369.00
ABI Human GAPD (GAPDH) Endogenous Control (FAM™ ⁄ MGB Probe, Non-Primer Limited) $330.00

 

  EPmotion Tips

EpMotion tips / card (15 x 96 tips) $280.00  
EpMotion tips / 1 x 96 tips $30 EPmotion Tips