KCCG operates Australia’s leading Oxford Nanopore research sequencing service. Nanopore sequencing is an emerging technology that enables sequencing of long, native DNA/RNA molecules, with no theoretical upper limit on read-length. Common research applications for nanopore sequencing include:
- Genome sequencing & assembly
- Resolving challenging genomic features, such as repetitive regions & structural variation
- Characterisation of full-length RNA isoforms & alternative splicing
- Detection of DNA/RNA base modifications, such as 5-methylcytosine
- Metagenomics & pathogen sequencing
We offer genome, transcriptome and metagenome sequencing projects of any size/specifications and pride ourselves on supporting custom research projects that push the boundaries. Find out more about our services.
The PromethION (right) is the largest instrument from Oxford Nanopore and enables comprehensive sequencing of large Eukaryotic genomes/transcriptomes. A single PromethION flow cell has 3,000 nanopore channels providing up to 12,000 pores for sequencing. We typically obtain ~50-100 Gigabases of sequenced reads from a single PromethION flow cell (~15-30X human genome coverage).
The GridION uses the same flow cell as the popular, portable MinION device, which has up to 512 nanopore channels and provides up to 2,048 pores. A GridION flow cell produces moderate outputs that are suitable for analysis of organisms with smaller genomes, such as microbial samples, or where shallow genome coverage is sufficient. We typically obtain ~8-20 Gigabases of sequenced output from a single GridION flow cell.
For small, rapid jobs, a third flow cell type is available. The Flongle flow cell has up to 126 pores and is useful for projects where only a small amount of data is required, such as sequencing of PCR amplicons or viral genomes. We typically obtain 0.1-1.0 Gigabases of sequenced output from a single Flongle flow cell.
The expected output from each of these platforms varies depending on sample quality, fragment sizes and other sample-specific variables. Please contact us for expected outputs for your sample type and recommendations on the appropriate platform.
KCCG strives to offer the cheapest pricing for nanopore sequencing in Australia. Please review our price guide below and email us to request a quote.
|PromethION flow cell||$2700|
|MinION / GridION flow cell||$900|
|DNA ligation library prep||$500|
|Direct RNA library prep||$560|
|Direct cDNA library prep||$580|
|Multiplexing (per sample)||$70|