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Next-Generation Sequencing of Antibody Display Repertoires

Abstract

In vitro selection technology has transformed the development of therapeutic monoclonal antibodies. Using methods such as phage, ribosome, and yeast display, high affinity binders can be selected from diverse repertoires. Here, we review strategies for the next-generation sequencing (NGS) of phage- and other antibody-display libraries, as well as NGS platforms and analysis tools. Moreover, we discuss recent examples relating to the use of NGS to assess library diversity, clonal enrichment, and affinity maturation.

Type Journal
ISBN 1664-3224 (Print) 1664-3224 (Linking)
Authors Rouet, R.; Jackson, K. J. L.; Langley, D. B.; Christ, D.
Responsible Garvan Author Dr Romain Rouet
Publisher Name Frontiers in Immunology
Published Volume 9
Published Pages 118
Status ePublication
DOI 10.3389/fimmu.2018.00118
URL link to publisher's version https://www.ncbi.nlm.nih.gov/pubmed/29472918