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I-motif DNA structures are formed in the nuclei of human cells

Abstract

Human genome function is underpinned by the primary storage of genetic information in canonical B-form DNA, with a second layer of DNA structure providing regulatory control. I-motif structures are thought to form in cytosine-rich regions of the genome and to have regulatory functions; however, in vivo evidence for the existence of such structures has so far remained elusive. Here we report the generation and characterization of an antibody fragment (iMab) that recognizes i-motif structures with high selectivity and affinity, enabling the detection of i-motifs in the nuclei of human cells. We demonstrate that the in vivo formation of such structures is cell-cycle and pH dependent. Furthermore, we provide evidence that i-motif structures are formed in regulatory regions of the human genome, including promoters and telomeric regions. Our results support the notion that i-motif structures provide key regulatory roles in the genome.

Type Journal
ISBN 1755-4349 (Electronic) 1755-4330 (Linking)
Authors Zeraati, M.; Langley, D. B.; Schofield, P.; Moye, A. L.; Rouet, R.; Hughes, W. E.; Bryan, T. M.; Dinger, M. E.; Christ, D.
Publisher Name Nature Chemistry
Published Date 2018-06-01
Published Volume 10
Published Issue 6
Published Pages 631-637
Status Published in-print
DOI 10.1038/s41557-018-0046-3
URL link to publisher's version https://www.ncbi.nlm.nih.gov/pubmed/29686376