I-motif DNA structures are formed in the nuclei of human cells
Human genome function is underpinned by the primary storage of genetic information in canonical B-form DNA, with a second layer of DNA structure providing regulatory control. I-motif structures are thought to form in cytosine-rich regions of the genome and to have regulatory functions; however, in vivo evidence for the existence of such structures has so far remained elusive. Here we report the generation and characterization of an antibody fragment (iMab) that recognizes i-motif structures with high selectivity and affinity, enabling the detection of i-motifs in the nuclei of human cells. We demonstrate that the in vivo formation of such structures is cell-cycle and pH dependent. Furthermore, we provide evidence that i-motif structures are formed in regulatory regions of the human genome, including promoters and telomeric regions. Our results support the notion that i-motif structures provide key regulatory roles in the genome.
|ISBN||1755-4349 (Electronic) 1755-4330 (Linking)|
|Authors||Zeraati, M.; Langley, D. B.; Schofield, P.; Moye, A. L.; Rouet, R.; Hughes, W. E.; Bryan, T. M.; Dinger, M. E.; Christ, D.|
|Responsible Garvan Author|
|Publisher Name||Nature Chemistry|
|URL link to publisher's version||https://www.ncbi.nlm.nih.gov/pubmed/29686376|
|OpenAccess link to author's accepted manuscript version||https://publications.gimr.garvan.org.au/open-access/14521|