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Expression of IgG Monoclonals with Engineered Immune Effector Functions


The therapeutic development of monoclonal antibodies requires robust and reliable methods for their recombinant expression and characterization. In this context, an increasingly important aspect in the antibody development process is to determine the contribution of Fc-mediated immune effector functions to therapeutic activity. Here we describe steps for the cloning and mammalian expression of mouse and human IgG monoclonals with reduced immune effector functions, based on mutation of Fc-gamma receptor and complement-binding sites. The resulting antibody preparations contain low levels of endotoxin and are suitable for testing in animal models of disease.

Type Book section
ISBN 1940-6029 (Electronic) 1064-3745 (Linking)
Authors Vazquez-Lombardi, R.; Nevoltris, D.; Rouet, R.; Christ, D.
Responsible Garvan Author Prof Daniel Christ
Publisher Name Methods in Molecular Biology
Published Date 2018-10-01
Published Volume 1827
Published Pages 313-334
Status Published in-print
DOI 10.1007/978-1-4939-8648-4_16
URL link to publisher's version