Selection of Antibody Fragments Against Structured DNA by Phage Display
In addition to the canonical B-form structure, DNA can adopt alternative conformations including Z DNA, triplex DNA, as well as G4 and i-Motif quadruplex structures. Such structures have been shown to form in cells in a dynamic manner. Monoclonal antibodies against such structures represent key tools to study the biological functions of these structures. Here we provide protocols for the generation of antibody fragments against structured DNA using phage display selections.
|ISBN||1940-6029 (Electronic) 1064-3745 (Linking)|
|Authors||Zeraati, M.; Dinger, M. E.; Christ, D.|
|Responsible Garvan Author||Prof Daniel Christ|
|Publisher Name||Methods in Molecular Biology|
|URL link to publisher's version||https://www.ncbi.nlm.nih.gov/pubmed/30196499|