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Selection of Antibody Fragments Against Structured DNA by Phage Display

Abstract

In addition to the canonical B-form structure, DNA can adopt alternative conformations including Z DNA, triplex DNA, as well as G4 and i-Motif quadruplex structures. Such structures have been shown to form in cells in a dynamic manner. Monoclonal antibodies against such structures represent key tools to study the biological functions of these structures. Here we provide protocols for the generation of antibody fragments against structured DNA using phage display selections.

Type Journal
ISBN 1940-6029 (Electronic) 1064-3745 (Linking)
Authors Zeraati, M.; Dinger, M. E.; Christ, D.
Responsible Garvan Author A/Prof Daniel Christ
Publisher Name Methods in Molecular Biology
Published Date 2018-10-01
Published Volume 1827
Published Pages 197-209
Status Published in-print
DOI 10.1007/978-1-4939-8648-4_11
URL link to publisher's version https://www.ncbi.nlm.nih.gov/pubmed/30196499