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Removing physiological motion from intravital and clinical functional imaging data


Intravital microscopy can provide unique insights into the function of biological processes in a native context. However, physiological motion caused by peristalsis, respiration and the heartbeat can present a significant challenge, particularly for functional readouts such as fluorescence lifetime imaging (FLIM), which require longer acquisition times to obtain a quantitative readout. Here, we present and benchmark Galene, a versatile multi-platform software tool for image-based correction of sample motion blurring in both time resolved and conventional laser scanning fluorescence microscopy data in two and three dimensions. We show that Galene is able to resolve intravital FLIM-FRET images of intra-abdominal organs in murine models and NADH autofluorescence of human dermal tissue imaging subject to a wide range of physiological motions. Thus, Galene can enable FLIM imaging in situations where a stable imaging platform is not always possible and rescue previously discarded quantitative imaging data.

Type Journal
ISBN 2050-084X (Electronic) 2050-084X (Linking)
Authors Warren, S. C.; Nobis, M.; Magenau, A.; Mohammed, Y. H.; Herrmann, D.; Moran, I.; Vennin, C.; Conway, J. R.; Melenec, P.; Cox, T. R.; Wang, Y.; Morton, J. P.; Welch, H. C.; Strathdee, D.; Anderson, K. I.; Phan, T. G.; Roberts, M. S.; Timpson, P.
Responsible Garvan Author (missing name)
Publisher Name eLife
Published Date 2018-07-09
Published Volume 7
Published Pages e35800
Status Always Electronic
DOI 10.7554/eLife.35800
URL link to publisher's version
OpenAccess link to author's accepted manuscript version