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Redefining tissue cross-talk via shotgun proteomic analyses of plasma extracellular vesicles

Abstract

Protein signalling between tissues, or tissue cross-talk is becoming recognised as a fundamental biological process that is incompletely understood. Shotgun proteomic analyses of tissues and plasma to explore this concept are regularly challenged by high dynamic range of protein abundance, which limits the identification of lower abundance proteins. In this viewpoint article, we highlight how a focus on proteins contained within extracellular vesicles (EVs) not only partially addresses this issue, it can reveal a underappreciated complexity of the circulating proteome in various physiological and pathological contexts. Furthermore, we highlight how quantitative proteomics can inform EV mediated cross talk and stress the importance of high coverage, sensitive proteomic analyses of EVs to identify both the optimal methods to isolate EV subtypes of interest and proteins that characterise them. This article is protected by copyright. All rights reserved.

Type Journal
ISBN 1615-9861 (Electronic) 1615-9853 (Linking)
Authors Whitham, M.; Febbraio, M. A.
Responsible Garvan Author Prof Mark Febbraio
Publisher Name PROTEOMICS
Published Date 2019-01-01
Published Pages e1800154
Status Published in-print
DOI 10.1002/pmic.201800154
URL link to publisher's version https://www.ncbi.nlm.nih.gov/pubmed/30350444