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Single-Cell Profiling Identifies Key Pathways Expressed by iPSCs Cultured in Different Commercial Media

Abstract

We assessed the pluripotency of human induced pluripotent stem cells (iPSCs) maintained on an automated platform using StemFlex and TeSR-E8 media. Analysis of transcriptome of single cells revealed similar expression of core pluripotency genes, as well as genes associated with naive and primed states of pluripotency. Analysis of individual cells from four samples consisting of two different iPSC lines each grown in the two culture media revealed a shared subpopulation structure with three main subpopulations different in pluripotency states. By implementing a machine learning approach, we estimated that most cells within each subpopulation are very similar between all four samples. The single-cell RNA sequencing analysis of iPSC lines grown in both media reports the molecular signature in StemFlex medium and how it compares to that observed in the TeSR-E8 medium.

Type Journal
ISBN 2589-0042 (Electronic) 2589-0042 (Linking)
Authors Daniszewski, M.; Nguyen, Q.; Chy, H. S.; Singh, V.; Crombie, D. E.; Kulkarni, T.; Liang, H. H.; Sivakumaran, P.; Lidgerwood, G. E.; Hernandez, D.; Conquest, A.; Rooney, L. A.; Chevalier, S.; Andersen, S. B.; Senabouth, A.; Vickers, J. C.; Mackey, D. A.; Craig, J. E.; Laslett, A. L.; Hewitt, A. W.; Powell, J. E.; Pebay, A.
Responsible Garvan Author Dr Joseph Powell
Publisher Name iScience
Published Date 2018-09-01
Published Volume 7
Published Pages 30-39
Status Always Electronic
DOI 10.1016/j.isci.2018.08.016
URL link to publisher's version https://www.ncbi.nlm.nih.gov/pubmed/30267684
OpenAccess link to author's accepted manuscript version https://publications.gimr.garvan.org.au/open-access/14835