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Protein Kinase C Epsilon Deletion in Adipose Tissue, but Not in Liver, Improves Glucose Tolerance

Abstract

Protein kinase C epsilon (PKCvarepsilon) activation in the liver is proposed to inhibit insulin action through phosphorylation of the insulin receptor. Here, however, we demonstrated that global, but not liver-specific, deletion of PKCvarepsilon in mice protected against diet-induced glucose intolerance and insulin resistance. Furthermore, PKCvarepsilon-dependent alterations in insulin receptor phosphorylation were not detected. Adipose-tissue-specific knockout mice did exhibit improved glucose tolerance, but phosphoproteomics revealed no PKCvarepsilon-dependent effect on the activation of insulin signaling pathways. Altered phosphorylation of adipocyte proteins associated with cell junctions and endosomes was associated with changes in hepatic expression of several genes linked to glucose homeostasis and lipid metabolism. The primary effect of PKCvarepsilon on glucose homeostasis is, therefore, not exerted directly in the liver as currently posited, and PKCvarepsilon activation in this tissue should be interpreted with caution. However, PKCvarepsilon activity in adipose tissue modulates glucose tolerance and is involved in crosstalk with the liver.

Type Journal
ISBN 1932-7420 (Electronic) 1550-4131 (Linking)
Authors Brandon, A. E.; Liao, B. M.; Diakanastasis, B.; Parker, B. L.; Raddatz, K.; McManus, S. A.; O'Reilly, L.; Kimber, E.; van der Kraan, A. G.; Hancock, D.; Henstridge, D. C.; Meikle, P. J.; Cooney, G. J.; James, D. E.; Reibe, S.; Febbraio, M. A.; Biden, T. J.; Schmitz-Peiffer, C.
Responsible Garvan Author A/Prof Carsten Schmitz-Peiffer
Publisher Name Cell Metabolism
Published Date 2019-01-08
Published Volume 29
Published Issue 1
Published Pages 183-191 e7
Status Published in-print
DOI 10.1016/j.cmet.2018.09.013
URL link to publisher's version https://www.ncbi.nlm.nih.gov/pubmed/30318338