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Droplet-based single cell RNAseq tools: a practical guide


Droplet based scRNA-seq systems such as Drop-seq, inDrop and Chromium 10X have been the catalyst for the wide adoption of high-throughput scRNA-seq technologies in the research laboratory. In order to understand the capabilities of these systems to deeply interrogate biology; here we provide a practical guide through all the steps involved in a typical scRNA-seq experiment. Through comparing and contrasting these three main droplet based systems (and their derivatives), we provide an overview of all critical considerations in obtaining high quality and biologically relevant data. We also discuss the limitations of these systems and how they fit into the emerging field of Genomic Cytometry.

Type Journal
ISBN 1473-0189 (Electronic) 1473-0189 (Linking)
Authors Salomon, R.; Kaczorowski, D.; Valdes-Mora, F.; Nordon, R. E.; Neild, A.; Farbehi, N.; Bartonicek, N.; Gallego-Ortega, D.
Responsible Garvan Author Dr David Gallego-Ortega
Publisher Name LAB ON A CHIP
Published Date 2019-05-14
Published Volume 19
Published Issue 10
Published Pages 1706-1727
Status Published in-print
DOI 10.1039/c8lc01239c
URL link to publisher's version