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Retention of paternal DNA methylome in the developing zebrafish germline

Abstract

Two waves of DNA methylation reprogramming occur during mammalian embryogenesis; during preimplantation development and during primordial germ cell (PGC) formation. However, it is currently unclear how evolutionarily conserved these processes are. Here we characterise the DNA methylomes of zebrafish PGCs at four developmental stages and identify retention of paternal epigenetic memory, in stark contrast to the findings in mammals. Gene expression profiling of zebrafish PGCs at the same developmental stages revealed that the embryonic germline is defined by a small number of markers that display strong developmental stage-specificity and that are independent of DNA methylation-mediated regulation. We identified promoters that are specifically targeted by DNA methylation in somatic and germline tissues during vertebrate embryogenesis and that are frequently misregulated in human cancers. Together, these detailed methylome and transcriptome maps of the zebrafish germline provide insight into vertebrate DNA methylation reprogramming and enhance our understanding of the relationships between germline fate acquisition and oncogenesis.

Type Journal
ISBN 2041-1723 (Electronic) 2041-1723 (Linking)
Authors Skvortsova, K.; Tarbashevich, K.; Stehling, M.; Lister, R.; Irimia, M.; Raz, E.; Bogdanovic, O.
Responsible Garvan Author Ozren Bogdanovic
Publisher Name Nature Communications
Published Date 2019-07-11
Published Volume 10
Published Issue 1
Published Pages 3054
Status Published in-print
DOI 10.1038/s41467-019-10895-6
URL link to publisher's version https://www.ncbi.nlm.nih.gov/pubmed/31296860