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Loss-of-function of the long non-coding RNA A830019P07Rik in mice does not affect insulin expression and secretion


Long non-coding RNAs (lncRNAs) contribute to diverse cellular functions and the dysregulation of their expression or function can contribute to diseases, including diabetes. The contributions of lncRNAs to beta-cell development, function and survival has been extensively studied in vitro. However, very little is currently known on the in vivo roles of lncRNAs in the regulation of glucose and insulin homeostasis. Here we investigated the impact of loss-of-function in mice of the lncRNA A830019P07Rik, hereafter P07Rik, which was previously reported to be associated with reduced plasma insulin levels. Compared with wild-type littermates, male and female P07Rik mutant mice did not show any defect in glycaemia and plasma insulin levels in both fed and fasted state. Furthermore, P07Rik mutant mice displayed similar glucose and insulin levels in response to an intra-peritoneal glucose tolerance test. Ex vivo, islets from mutant P07Rik released similar amount of insulin in response to increased glucose concentration as wildtype littermates. In contrast with previous reports, our characterization of P07Rik mouse mutants revealed that loss of function of this lncRNA does not affect glucose and insulin homeostasis in mice.

Type Journal
ISBN 2045-2322 (Electronic) 2045-2322 (Linking)
Authors Guay, C.; Abdulkarim, B.; Tan, J. Y.; Dubuis, G.; Rutti, S.; Ross Laybutt, D.; Widmann, C.; Regazzi, R.; Marques, A. C.
Responsible Garvan Author A/Prof Ross Laybutt
Publisher Name Scientific Reports
Published Date 2020-04-14
Published Volume 10
Published Issue 1
Published Pages 6413
Status Published in-print
DOI 10.1038/s41598-020-62969-x
URL link to publisher's version