Publications Search

Search for publications by author
Search for publications by abstract keyword(s)

Spatial and Temporal Control of CRISPR-Cas9-Mediated Gene Editing Delivered via a Light-Triggered Liposome System


The CRISPR-Cas9 and related systems offer a unique genome-editing tool allowing facile and efficient introduction of heritable and locus-specific sequence modifications in the genome. Despite its molecular precision, temporal and spatial control of gene editing with the CRISPR-Cas9 system is very limited. We developed a light-sensitive liposome delivery system that offers a high degree of spatial and temporal control of gene editing with the CRISPR-Cas9 system. We demonstrated its efficient protein release by respectively assessing the targeted knockout of the eGFP gene in human HEK293/GFP cells and the TNFAIP3 gene in TNFalpha-induced HEK293 cells. We further validated our results at a single-cell resolution using an in vivo eGFP reporter system in zebrafish (77% knockout). These findings indicate that light-triggered liposomes may have new options for precise control of CRISPR-Cas9 release and editing.

Type Journal
ISBN 1944-8252 (Electronic) 1944-8244 (Linking)
Authors Aksoy, Y. A.; Yang, B.; Chen, W.; Hung, T.; Kuchel, R. P.; Zammit, N. W.; Grey, S. T.; Goldys, E. M.; Deng, W.
Responsible Garvan Author (missing name)
Publisher Name ACS Applied Materials & Interfaces
Published Date 2020-11-30
Published Volume 12
Published Issue 47
Published Pages 52433-52444
Status Published in-print
DOI 10.1021/acsami.0c16380
URL link to publisher's version