Publications Search

Search for publications by author
Search for publications by abstract keyword(s)

The emergence of the brain non-CpG methylation system in vertebrates


Mammalian brains feature exceptionally high levels of non-CpG DNA methylation alongside the canonical form of CpG methylation. Non-CpG methylation plays a critical regulatory role in cognitive function, which is mediated by the binding of MeCP2, the transcriptional regulator that when mutated causes Rett syndrome. However, it is unclear whether the non-CpG neural methylation system is restricted to mammalian species with complex cognitive abilities or has deeper evolutionary origins. To test this, we investigated brain DNA methylation across 12 distantly related animal lineages, revealing that non-CpG methylation is restricted to vertebrates. We discovered that in vertebrates, non-CpG methylation is enriched within a highly conserved set of developmental genes transcriptionally repressed in adult brains, indicating that it demarcates a deeply conserved regulatory program. We also found that the writer of non-CpG methylation, DNMT3A, and the reader, MeCP2, originated at the onset of vertebrates as a result of the ancestral vertebrate whole-genome duplication. Together, we demonstrate how this novel layer of epigenetic information assembled at the root of vertebrates and gained new regulatory roles independent of the ancestral form of the canonical CpG methylation. This suggests that the emergence of non-CpG methylation may have fostered the evolution of sophisticated cognitive abilities found in the vertebrate lineage.

Type Journal
ISBN 2397-334X (Electronic) 2397-334X (Linking)
Authors de Mendoza, A.; Poppe, D.; Buckberry, S.; Pflueger, J.; Albertin, C. B.; Daish, T.; Bertrand, S.; de la Calle-Mustienes, E.; Gomez-Skarmeta, J. L.; Nery, J. R.; Ecker, J. R.; Baer, B.; Ragsdale, C. W.; Grutzner, F.; Escriva, H.; Venkatesh, B.; Bogdanovic, O.; Lister, R.
Responsible Garvan Author A/Prof Ozren Bogdanovic
Publisher Name Nature Ecology & Evolution
Published Date 2021-03-31
Published Volume 5
Published Issue 3
Published Pages 369-378
Status Published in-print
DOI 10.1038/s41559-020-01371-2
URL link to publisher's version