The BD FACSymphony is a high throughput analysing cytometer that has eight excitation lasers:

• UV 355 nm
• Violet 402 nm
• Blue 488 nm
• Blue/Violet 442 nm
• Yellow/green 561nm
• Red (628 nm)/Orange (592 nm)
• NIR 785 nm

• The FACSymphony is our newest analysing cytometer with the capacity to analyse 50 colours using the most cutting edge technology BD has to offer. Compared to alternative cell analysis system , the BD FACSymphony instrument offers high-speed analysis with detection rates of up to 40000 events per second.
• This advanced platform features an ultra-quiet electronics system adapted that improves detection sensitivity to enable the identification and analysis of rare cell types and events.
• Please contact gwccgfacility@garvan.org.au for training and analaysis.
• Currently running on FACSDiva 8.0.
• For more info, please visit  BD FACSymphony™ A5 Cell Analyzer brochure. 

The BD LSR Fortessa is a 4 Laser Cell Analyser with ability to detect up to 18 parameters (16 fluorochromes + Side scatter+ forward scatter) to run a comprehensive multi-colour flow cytometry assay. 

Excitation lasers:

• Violet 405 nm
• Blue 488 nm
• Yellow/Green 561 nm
• Red 633 nm
• UV 355nm

•  The Fortessa runs on FACS Diva 8 for data aquisition and analysis.
• For more info, please visit BD LSRFortessa™ System brochure.

The BD LSR SORP is a high end cell analysing cytometer that has five excitation lasers (discontinued):

• UV355nm
• Violet 405nm
• Blue 488nm
• Yellow/green 561nm
• Red 640nm

• The SORP is our most popular cell analysis instrument as it has a solid solid state UV laser that can detect dyes with low excitation wavelengths (e.g. Hoescht, DAPI and Indo-1). The addition of the UV lasers enables the user to expand their fluorochrome panel to up to 19 (17 fluorochromes + Forward Scatter + Side Scatter).
• The SORP also has a green laser (532nm) that can provide even more flexibility with designing a robust fluorochrome panel with minimal spectral overlap. Please contact us if you require the green laser in your experiments.
• Runs on FACS Diva 8.
• The instrument is now discontinued from BD.

The BD FACSCanto^TM II system is a compact flow cytometer that allows quick sample acquisition, fully automated fluidics start up that is designed to streamline work, save time, and improve performance.

The FACS Canto II has 3 excitation lasers:

• Violet 405nm
• Blue 488nm
• Red 633nm

• The Canto II is capable of detecting up to 10 parameters (8 fluorochromes + Forward Scatter + Side Scatter).
• The Canto II runs on FACS Diva 8.
• For more info, please visit D FACSCanto™ II System brochure.

The Beckman Coulter Cytoflex is a compact benchtop flow cytometer that is equipped with 3 lasers and can detect up to 13 parameters (including forward/side scatter). 

Excitation lasers:

• Violet 405nm
• Blue 488nm
• Red 640nm

• This instrument features a fully automated start up procedure and innovative fluidics system that minimises waste and sheath required for acquisition. The Cytoflex tube loader acquires samples from FACS tubes.
• The Cytoflex plate reader is equipped with the CytExpert Software program mentioned on the tube analyser page which has features such as auto-thresholding and a compensation library for quick access to previously stored compensation matrices.

• The Cytoflex software 'CytExpert' has numerous useful features:

  • A compensation library to store a repository of compensation control panels in a libraryfor easy access to the correct compensation matrix with new gain settings.
  • Auto thresholding to easily find target populations. Manual thresholding can also be selected.
  • Axis Pan which is an ideal tool for visualizing data across a high dynamic range axis. You can easily pan across the axis range to search if there is important data out of range.