Yes we do. Our sequencing services are readily available worldwide.
We have extensive experience in providing whole genome sequencing (WGS) and now, with the recent validation of the NovaSeq 6000, can apply our expertise and laboratory skills to a broader range of sequencing to suit your projects needs.
Services cover a range of sequencing applications including;
- Whole Genome Sequencing
- Whole Exome Sequencing
- Targeted Sequencing
- Single-cell RNA-Seq
- DeNovo Sequencing
- Linked-read sequencing
We are happy to discuss other applications of the sequencing service to try and fit your needs.
Researchers are given access to an online electronic manifest into which several sample characteristics, required for the processing of the sample, must be entered. The manifest is pre-filled with a barcode corresponding to each FluidX 2D tube provided for your samples. The barcode provides a unique identification code for each submitted sample.
Data will be delivered via DNAnexus, a cloud-based platform. Your data will be shipped with a detailed project summary report describing the yield and quality of sequence data for each sample. With your data, you will also find documentation that describes the file structure of your sequencing data and a project summary report. In accordance with our terms and conditions, we guarantee yield ≥100Gb and Q30 ≥75% of bases.
These data are from the Coriell Cell Repository NA12878 reference cell line, which has been extensively analysed by the Genome in a Bottle Consortium. The sequencing libraries were generated with Illumina’s TruSeq Nano V2.5 kit using the Hamilton Microlab STAR robotics platform, achieving >400bp inserts. Each library was sequenced on a single lane of an Illumina HiSeq X patterned flow cell, achieving over 130Gb of yield, with > 83% of bases with quality > Q30 in just 2.8 days. The four data sets are of similar quality, and are provided to allow you to assess the reproducibility of the technology. Each data set substantially surpasses the minimum coverage and quality guaranteed by Illumina and is indicative of the potential for the Illumina HiSeq X Ten sequencing system.
Each of the four datasets consists of raw paired-end data (fastq.gz files) and results obtained with the GATK DNAseq best practices pipeline run on each library independently with the recommended parameters for whole genome sequencing.
Download the data to your computer or server, using the links below.
Human Sample Data
|Read length||151bp PE||151bp PE||151bp PE||151bp PE|
|Raw Read Pairs (PF)||439,013,514||510,726,469||464,350,208||479,861,658|
|Raw Yield (Gb)||131.704||153.218||139.305||143.958|
|% bases >=Q30 (R1/R2)||92.39/81.23||93.18/73.37||89.89/77.44||93.00/78.75|
|% bases >=Q30 (mean)||86.81||83.28||83.67||85.88|
We have flexible payment options available. Please contact us to discuss further.