Real time intraoperative confocal laser microscopy-guided surgery
OBJECTIVE: To assess the potential utility of in vivo histologic surface and subsurface imaging in real-time using the Optiscan confocal laser microscope to detect diseased tissue at the time of surgery. SUMMARY BACKGROUND DATA: The goal of surgical treatment of diseases such as cancer is complete microscopic resection of diseased tissue; however, current methods for the assessment of extent of disease at the time of surgery are inadequate. METHODS: We assessed the potential of the Optiscan confocal laser microscope, a new device developed for real-time in vivo histologic surface and subsurface imaging during surgery. RESULTS: Intravenous Fluorescein Sodium contrast enabled visualization of cellular and architectural morphology of intra-abdominal organs with magnification equivalent to light microscopy and enabled differentiation between normal organs and disease. CONCLUSIONS: Real time intraoperative confocal microscopy has significant potential application in detecting disease, and influencing decision-making at the time of surgery.
|Authors||Nguyen, N. Q.; Biankin, A. V.; Leong, R. W.; Chang, D. K.; Cosman, P. H.; Delaney, P.; Kench, J. G.; Merrett, N. D.;|
|Publisher Name||ANNALS OF SURGERY|
|URL link to publisher's version||http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19387332|
|OpenAccess link to author's accepted manuscript version||https://publications.gimr.garvan.org.au/open-access/10227|