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Consolidation of the cancer genome into domains of repressive chromatin by long-range epigenetic silencing (LRES) reduces transcriptional plasticity


Silencing of individual genes can occur by genetic and epigenetic processes during carcinogenesis, but the underlying mechanisms remain unclear. By creating an integrated prostate cancer epigenome map using tiling arrays, we show that contiguous regions of gene suppression commonly occur through long-range epigenetic silencing (LRES). We identified 47 LRES regions in prostate cancer, typically spanning about 2 Mb and harbouring approximately 12 genes, with a prevalence of tumour suppressor and miRNA genes. Our data reveal that LRES is associated with regional histone deacetylation combined with subdomains of different epigenetic remodelling patterns, which include re-enforcement, gain or exchange of repressive histone, and DNA methylation marks. The transcriptional and epigenetic state of genes in normal prostate epithelial and human embryonic stem cells can play a critical part in defining the mode of cancer-associated epigenetic remodelling. We propose that consolidation or effective reduction of the cancer genome commonly occurs in domains through a combination of LRES and LOH or genomic deletion, resulting in reduced transcriptional plasticity within these regions.

Type Journal
ISBN 1476-4679 (Electronic) 1465-7392 (Linking)
Authors Coolen, M.W., Stirzaker, C., Song, J.Z., Statham, A.L., Kassir, Z., Moreno, C.S., Young, A.N., Varma, V., Speed, T.P., Cowley, M., Lacaze, P., Kaplan, W., Robinson, M.D.; Clark, S.J.
Garvan Authors Dr Mark Cowley , Prof Susan Clark , Dr Warren Kaplan
Publisher Name NAT CELL BIOL
Published Date 2010-03-01 00:00:00
Published Volume 12
Published Issue 3
Published Pages 235
Status Published In-print
OpenAccess Link