Follicular helper T cell differentiation requires continuous antigen presentation that is independent of unique B cell signaling
Effective humoral immunity depends on the support of B cell responses by T follicular helper (Tfh) cells. Although it has been proposed that Tfh cell differentiation requires T-B interactions, the relative contribution of specific populations of Ag-presenting cells remains unknown. We employed three independent strategies that compromised interactions between CD4(+) T cells and activated B cells in vivo. Whereas the expansion of CD4(+) T cells was relatively unaffected, Tfh cell differentiation was completely blocked in all scenarios. Surprisingly, augmenting antigen presentation by non-B cells rescued Tfh cell differentiation, as determined by surface phenotype, gene expression, and germinal center localization. We conclude that although Ag presentation by responding B cells is typically required for the generation of Tfh cells, this does not result from the provision of a unique B cell-derived signal, but rather because responding B cells rapidly become the primary source of antigen.
|ISBN||1097-4180 (Electronic) 1074-7613 (Linking)|
|Authors||Deenick, E. K.; Chan, A.; Ma, C. S.; Gatto, D.; Schwartzberg, P. L.; Brink, R.; Tangye, S. G.|
|DOI||S1074-7613(10)00280-3 [pii] 10.1016/j.immuni.2010.07.015|
|URL link to publisher's version||http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=20691615|
|OpenAccess link to author's accepted manuscript version||https://publications.gimr.garvan.org.au/open-access/10525|