Publications

Publications Search

Search for publications by author
Search for publications by abstract keyword(s)

IL-27 supports germinal center function by enhancing IL-21 production and the function of T follicular helper cells

Abstract

Maturation and selection of high-affinity B cell clones in the germinal center (GC) relies on support from T follicular helper (T(FH)) cells. T(FH) cells are characterized by their localization to the B cell follicle and their high expression of the costimulatory molecules ICOS and PD1 and the cytokine IL-21, which promotes immunoglobulin (Ig) class switching and production by B cells. We show that the heterodimeric cytokine IL-27 is critical for the function of T(FH) cells and for normal and pathogenic GC responses. IL-27 signaling to T cells results in the production of IL-21, a known autocrine factor for the maintenance of T(FH) cells, in a STAT3-dependent manner. IL-27 also enhances the survival of activated CD4(+) T cells and the expression of T(FH) cell phenotypic markers. In vivo, expression of the IL-27Ralpha chain is required to support IL-21 production and T(FH) cell survival in a T cell-intrinsic manner. The production of high-affinity antibodies is reduced, and pristane-elicited autoantibodies and glomerulonephritis are significantly diminished, in Il27ra(-/-) mice. Together, our data show a nonredundant role for IL-27 in the development of T cell-dependent antibody responses.

Type Journal
ISBN 0022-1007 (Linking) 0022-1007 (Linking)
Authors Batten, M.; Ramamoorthi, N.; Kljavin, N. M.; Ma, C. S.; Cox, J. H.; Dengler, H. S.; Danilenko, D. M.; Caplazi, P.; Wong, M.; Fulcher, D. A.; Cook, M. C.; King, C.; Tangye, S. G.; de Sauvage, F. J.; Ghilardi, N.:
Responsible Garvan Author Dr Marcel Batten
Publisher Name JOURNAL OF EXPERIMENTAL MEDICINE
Published Date 2010-11-22
Published Volume 207
Published Issue 13
Published Pages 2895-906
Status Published in-print
DOI jem.20100064 [pii] 10.1084/jem.20100064
URL link to publisher's version http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=21098093
OpenAccess link to author's accepted manuscript version https://publications.gimr.garvan.org.au/open-access/10530