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Exocytotic vesicle behaviour assessed by total internal reflection fluorescence microscopy


The regulated trafficking or exocytosis of cargo-containing vesicles to the cell surface is fundamental to all cells. By coupling the technology of fluorescently tagged fusion proteins with total internal reflection fluorescence microscopy (TIRFM), it is possible to achieve the high spatio-temporal resolution required to study the dynamics of sub-plasma membrane vesicle trafficking and exocytosis. TIRFM has been used in a number of cell types to visualize and dissect the various steps of exocytosis revealing how molecules identified via genetic and/or biochemical approaches are involved in the regulation of this process. Here, we summarize the contribution of TIRFM to our understanding of the mechanism of exocytosis and discuss the novel methods of analysis that are required to exploit the large volumes of data that can be produced using this technique.

Type Journal
ISBN 1398-9219 (Linking)
Authors Burchfield, J. G.; Lopez, J. A.; Mele, K.; Vallotton, P.; Hughes, W. E.;
Publisher Name TRAFFIC
Published Date 2010-01-01
Published Volume 11
Published Issue 4
Published Pages 429-39
Status Published in-print
URL link to publisher's version
OpenAccess link to author's accepted manuscript version