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Discovery pipeline for epigenetically deregulated miRNAs in cancer: integration of primary miRNA transcription


BACKGROUND: Cancer is commonly associated with widespread disruption of DNA methylation, chromatin modification and miRNA expression. In this study, we established a robust discovery pipeline to identify epigenetically deregulated miRNAs in cancer. RESULTS: Using an integrative approach that combines primary transcription, genome-wide DNA methylation and H3K9Ac marks with microRNA (miRNA) expression, we identified miRNA genes that were epigenetically modified in cancer. We find miR-205, miR-21, and miR-196b to be epigenetically repressed, and miR-615 epigenetically activated in prostate cancer cells. CONCLUSIONS: We show that detecting changes in primary miRNA transcription levels is a valuable method for detection of local epigenetic modifications that are associated with changes in mature miRNA expression.

Authors Hulf, T.; Sibbritt, T.; Wiklund, E. D.; Bert, S.; Strbenac, D.; Statham, A. L.; Robinson, M. D.; Clark, S. J.
Publisher Name BMC Genomics
Published Date 2011-03-01
Published Volume 12
Published Pages 54
Status Published in-print
DOI 1471-2164-12-54 [pii] 10.1186/1471-2164-12-54
URL link to publisher's version
OpenAccess link to author's accepted manuscript version