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TIRF microscopy software for automated vesicle fusion detection


The recent explosion in the use of total internal reflection fluorescence (TIRF) microscopy to learn about cell trafficking means that there is an urgent need for software capable of annotating the corresponding movies automatically. TIRF Explorer software (CSIRO and Garvan, NSW, Australia) speeds up identification of vesicle?plasma membrane fusion events, a notoriously time-consuming task (see Figure 1). The authors? results indicate that it is possible to detect the majority of events automatically and that the rate of false positive and missed events compares well to what can be achieved by a human expert. The system is particularly useful to researchers in the areas of diabetes and obesity.

Type Journal
ISBN 0749-3223
Authors Mele, K.; Burchfield, J. G.; Lopez, J. A.; James, D. E.; Hughes, W. E.; Coster, A.; Vallotton, P.;
Publisher Name American Biotechnology Laboratory
Published Date 2009-01-01 00:00:00
Published Volume 27
Published Issue 10
Published Pages 8-9
Status Published In-print
OpenAccess Link Mele, K ABL.pdf