TIRF microscopy software for automated vesicle fusion detection
The recent explosion in the use of total internal reflection fluorescence (TIRF) microscopy to learn about cell trafficking means that there is an urgent need for software capable of annotating the corresponding movies automatically. TIRF Explorer software (CSIRO and Garvan, NSW, Australia) speeds up identification of vesicle?plasma membrane fusion events, a notoriously time-consuming task (see Figure 1). The authors? results indicate that it is possible to detect the majority of events automatically and that the rate of false positive and missed events compares well to what can be achieved by a human expert. The system is particularly useful to researchers in the areas of diabetes and obesity.
|Authors||Mele, K.; Burchfield, J. G.; Lopez, J. A.; James, D. E.; Hughes, W. E.; Coster, A.; Vallotton, P.;|
|Responsible Garvan Author|
|Publisher Name||American Biotechnology Laboratory|
|URL link to publisher's version||http://www.nxtbook.com/nxtbooks/isc/abl_200910/index.php?startid=13#/0|
|OpenAccess link to author's accepted manuscript version||https://publications.gimr.garvan.org.au/open-access/10735|