Publications Search

Search for publications by author
Search for publications by abstract keyword(s)

Expression of high affinity human antibody fragments in bacteria


Here we describe protocols for the expression of human antibody fragments in Escherichia Coli. Antigen-specific clones are identified by soluble fragment ELISA and concentrated by periplasmic preparation. They are then further purified by affinity chromatography. This article provides an overview of expression and purification strategies for human antibody fragments, as well as detailed protocols for the identification of high affinity binders and for affinity maturation.

Type Journal
Authors Rouet, R.; Lowe, D.; Dudgeon, K.; Roome, B.; Schofield, P.; Langley, D.; Andrews, J.; Whitfeld, P.; Jermutus, L.; Christ, D.:
Responsible Garvan Author (missing name)
Publisher Name Nature Protocols
Published Date 2012-02-02
Published Volume 7
Published Issue 2
Published Pages 364-73
Status Published in-print
DOI 10.1038/nprot.2011.448
URL link to publisher's version
OpenAccess link to author's accepted manuscript version