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Generation of human single domain antibody repertoires by kunkel mutagenesis

Abstract

Human antibody single domains are a promising new class of antibody fragments. Here we describe methods for the cloning of human V(H) and V(L) genes into phage and phagemid vectors. Furthermore, we provide detailed protocols for the generation of single domain antibody libraries by Kunkel mutagenesis and the analysis of diversity by DNA sequencing and superantigen binding.

Type Journal
ISBN 1940-6029 (Electronic) 1064-3745 (Linking)
Authors Rouet, R.; Dudgeon, K.; Christ, D.;
Responsible Garvan Author A/Prof Daniel Christ
Publisher Name Methods Mol Biol
Published Date 2012-01-01
Published Volume 907
Published Pages 195-209
Status Published in-print
URL link to publisher's version http://www.ncbi.nlm.nih.gov/pubmed/22907352
OpenAccess link to author's accepted manuscript version https://publications.gimr.garvan.org.au/open-access/11673