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Generation of human single domain antibody repertoires by kunkel mutagenesis


Human antibody single domains are a promising new class of antibody fragments. Here we describe methods for the cloning of human V(H) and V(L) genes into phage and phagemid vectors. Furthermore, we provide detailed protocols for the generation of single domain antibody libraries by Kunkel mutagenesis and the analysis of diversity by DNA sequencing and superantigen binding.

Type Journal
ISBN 1940-6029 (Electronic) 1064-3745 (Linking)
Authors Rouet, R.; Dudgeon, K.; Christ, D.;
Garvan Authors A/Prof Daniel Christ
Publisher Name Methods Mol Biol
Published Date 2012-01-01 00:00:00
Published Volume 907
Published Pages 195-209
Status Published in-print