Publications

Publications Search

Search for publications by author
Search for publications by abstract keyword(s)

Dynamic adipocyte phosphoproteome reveals that Akt directly regulates mTORC2

Abstract

A major challenge of the post-genomics era is to define the connectivity of protein phosphorylation networks. Here, we quantitatively delineate the insulin signaling network in adipocytes by high-resolution mass spectrometry-based proteomics. These data reveal the complexity of intracellular protein phosphorylation. We identified 37,248 phosphorylation sites on 5,705 proteins in this single-cell type, with approximately 15% responding to insulin. We integrated these large-scale phosphoproteomics data using a machine learning approach to predict physiological substrates of several diverse insulin-regulated kinases. This led to the identification of an Akt substrate, SIN1, a core component of the mTORC2 complex. The phosphorylation of SIN1 by Akt was found to regulate mTORC2 activity in response to growth factors, revealing topological insights into the Akt/mTOR signaling network. The dynamic phosphoproteome described here contains numerous phosphorylation sites on proteins involved in diverse molecular functions and should serve as a useful functional resource for cell biologists.

Type Journal
Authors Humphrey, S. J.; Yang, G.; Yang, P.; Fazakerley, D. J.; Stockli, J.; Yang, J. Y.; James, D. E.
Responsible Garvan Author
Publisher Name Cell Metabolism
Published Date 2013-06-16
Published Volume 17
Published Issue 6
Published Pages 1009-20
Status Published in-print
DOI 10.1016/j.cmet.2013.04.010
URL link to publisher's version http://www.ncbi.nlm.nih.gov/pubmed/23684622
OpenAccess link to author's accepted manuscript version https://publications.gimr.garvan.org.au/open-access/11791