Transitional B cell subsets in human bone marrow
B cells originate from precursors in the bone marrow, and the first cells which migrate to the peripheral blood have been classified as 'transitional B cells'. Transitional B cells have been characterized in human blood with stage 1 (T1) and stage 2 (T2) subsets being proposed. In the present study, 27 normal human bone marrow samples were analysed for transitional B cell markers by eight-colour flow cytometry. T1 transitional B cells (CD45(+) CD19(+) CD10(+) IgM(+) IgD(lo) ) and T2 transitional B cells (CD45(+) CD19(+) CD10(+) IgM(+) IgD(+) ) were identified in normal bone marrow samples at a mean frequency of 3.2 and 3.1% of total B lineage cells, respectively. A majority of the bone marrow transitional B cells were CD24(hi) CD38(hi) , the phenotype of blood transitional B cells. Consistent with recent peripheral blood data, T2 B cells had a significantly higher CD21 expression compared with T1 B cells (72.4 versus 40.9%) in the bone marrow. These data raise the possibility that transitional B cells are capable of differentiating from T1 to T2 B cells within the bone marrow. Furthermore, transitional cells at either stages 1 or 2 might be capable of migrating out of the bone marrow.
|Authors||Agrawal, S.; Smith, S. A.; Tangye, S. G.; Sewell, W. A.|
|Responsible Garvan Author||Prof Bill Sewell|
|Publisher Name||CLINICAL AND EXPERIMENTAL IMMUNOLOGY|
|URL link to publisher's version||http://www.ncbi.nlm.nih.gov/pubmed/23731328|
|OpenAccess link to author's accepted manuscript version||https://publications.gimr.garvan.org.au/open-access/11817|