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Mapping of mitochondrial RNA-protein interactions by digital RNase footprinting


Human mitochondrial DNA is transcribed as long polycistronic transcripts that encompass each strand of the genome and are processed subsequently into mature mRNAs, tRNAs, and rRNAs, necessitating widespread posttranscriptional regulation. Here, we establish methods for massively parallel sequencing and analyses of RNase-accessible regions of human mitochondrial RNA and thereby identify specific regions within mitochondrial transcripts that are bound by proteins. This approach provides a range of insights into the contribution of RNA-binding proteins to the regulation of mitochondrial gene expression.

Type Journal
Authors Liu, G.; Mercer, T. R.; Shearwood, A. M.; Siira, S. J.; Hibbs, M. E.; Mattick, J. S.; Rackham, O.; Filipovska, A.
Publisher Name Cell Reports
Published Date 2013-11-14
Published Volume 5
Published Issue 3
Published Pages 839-48
Status Published in-print
DOI 10.1016/j.celrep.2013.09.036
URL link to publisher's version
OpenAccess link to author's accepted manuscript version