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Redemption of autoantibodies on anergic B cells by variable-region glycosylation and mutation away from self-reactivity


The best-understood mechanisms for achieving antibody self/non-self discrimination discard self-reactive antibodies before they can be tested for binding microbial antigens, potentially creating holes in the repertoire. Here we provide evidence for a complementary mechanism: retaining autoantibodies in the repertoire displayed as low levels of IgM and high IgD on anergic B cells, masking a varying proportion of autoantibody-binding sites with carbohydrates, and removing their self-reactivity by somatic hypermutation and selection in germinal centers (GCs). Analysis of human antibody sequences by deep sequencing of isotype-switched memory B cells or in IgG antibodies elicited against allogeneic RhD+ erythrocytes, vaccinia virus, rotavirus, or tetanus toxoid provides evidence for reactivation of anergic IgM(low) IgD+ IGHV4-34+ B cells and removal of cold agglutinin self-reactivity by hypermutation, often accompanied by mutations that inactivated an N-linked glycosylation sequon in complementarity-determining region 2 (CDR2). In a Hy10 antibody transgenic model where anergic B cells respond to a biophysically defined lysozyme epitope displayed on both foreign and self-antigens, cell transfers revealed that anergic IgM(low) IgD+ B cells form twice as many GC progeny as naive IgM(hi) IgD+ counterparts. Their GC progeny were rapidly selected for CDR2 mutations that blocked 72% of antigen-binding sites with N-linked glycan, decreased affinity 100-fold, and then cleared the binding sites of blocking glycan. These results provide evidence for a mechanism to acquire self/non-self discrimination by somatic mutation away from self-reactivity, and reveal how varying the efficiency of N-glycosylation provides a mechanism to modulate antibody avidity.

Type Journal
ISBN 1091-6490 (Electronic) 0027-8424 (Linking)
Authors Sabouri, Z. ; Schofield, P. ; Horikawa, K. ; Spierings, E. ; Kipling, D. ; Randall, K. L. ; Langley, D. ; Roome, B. ; Vazquez-Lombardi, R. ; Rouet, R. ; Hermes, J. ; Chan, T. D. ; Brink, R. ; Dunn-Walters, D. K. ; Christ, D. ; Goodnow, C. C.;
Published Date 2014-01-01
Published Volume 111
Published Issue 25
Published Pages E2567-75
Status Published in-print
URL link to publisher's version
OpenAccess link to author's accepted manuscript version