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Methylation-capture and Next-Generation Sequencing of free circulating DNA from human plasma


BACKGROUND: Free circulating DNA (fcDNA) has many potential clinical applications, due to the non-invasive way in which it is collected. However, because of the low concentration of fcDNA in blood, genome-wide analysis carries many technical challenges that must be overcome before fcDNA studies can reach their full potential. There are currently no definitive standards for fcDNA collection, processing and whole-genome sequencing. We report novel detailed methodology for the capture of high-quality methylated fcDNA, library preparation and downstream genome-wide Next-Generation Sequencing. We also describe the effects of sample storage, processing and scaling on fcDNA recovery and quality. RESULTS: Use of serum versus plasma, and storage of blood prior to separation resulted in genomic DNA contamination, likely due to leukocyte lysis. Methylated fcDNA fragments were isolated from 5 donors using a methyl-binding protein-based protocol and appear as a discrete band of ~180 bases. This discrete band allows minimal sample loss at the size restriction step in library preparation for Next-Generation Sequencing, allowing for high-quality sequencing from minimal amounts of fcDNA. Following sequencing, we obtained 37x106-86x106 unique mappable reads, representing more than 50% of total mappable reads. The methylation status of 9 genomic regions as determined by DNA capture and sequencing was independently validated by clonal bisulphite sequencing. CONCLUSIONS: Our optimized methods provide high-quality methylated fcDNA suitable for whole-genome sequencing, and allow good library complexity and accurate sequencing, despite using less than half of the recommended minimum input DNA.

Type Journal
ISBN 1471-2164 (Electronic) 1471-2164 (Linking)
Authors Warton, K.; Lin, V.; Navin, T.; Armstrong, N. J.; Kaplan, W.; Ying, K.; Gloss, B.; Mangs, H.; Nair, S. S.; Hacker, N. F.; Sutherland, R. L.; Clark, S. J.; Samimi, G.;
Garvan Authors Dr Brian Gloss , Kevin Ying , Dr Kristina Warton , Dr Nicola Armstrong , Dr Shalima Nair , Prof Susan Clark , Dr Warren Kaplan
Publisher Name BMC GENOMICS
Published Date 2014-01-01 00:00:00
Published Volume 15
Published Pages 476
Status Published In-print
OpenAccess Link